English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/116317
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
DC FieldValueLanguage
dc.contributor.authorPérez, Pilar-
dc.contributor.authorRibas, Juan Carlos-
dc.date.accessioned2015-06-09T11:49:13Z-
dc.date.available2015-06-09T11:49:13Z-
dc.date.issued2013-
dc.identifierdoi: 10.1007/978-1-4614-2356-0_12-
dc.identifierisbn: 978-1-4614-2355-3-
dc.identifier.citationLaboratory Protocols in Fungal Biology: Current Methods in Fungal Biology: 175-196 (2013)-
dc.identifier.urihttp://hdl.handle.net/10261/116317-
dc.description.abstractFungal cell wall is a rigid structure mainly composed of polysaccharides (up to 90 %) and glycoproteins. It is essential for survival of the fungal cells, because it protects them against bursting caused by internal turgor pressure and against mechanical injury. Because of its absence in mammalian cells, it is an attractive target for antifungal agents. Thus, for various reasons, it might be important to know how the cell wall is synthesized, and how to analyze its composition. We provide here information about in vitro analysis of the biosynthetic activities of the main fungal wall and describe some methods for rapid analysis of cell wall composition by using specific enzymatic degradations. We also describe some additional methods that can be occasionally used to analyze fungal wall properties or composition. These methods provide powerful tools to evaluate changes in fungal cell walls and will be useful for screening new compounds for antifungal activity that might cause inhibition of cell wall biosynthesis and/or alter the structure of the fungal cell wall.-
dc.description.sponsorshipThis work was supported by grants BFU2010-15641 and BIO2009-10597 from the Dirección General de Investigación, MICINN, Spain, and grant CSI038A11-2 from the Junta de Castilla y León, Spain.-
dc.publisherSpringer-
dc.rightsclosedAccess-
dc.subjectGlucan-
dc.subjectPolysaccharides-
dc.subjectChitin-
dc.subjectMannan-
dc.subjectAntifungal drugs-
dc.subjectCell wall-
dc.titleFungal cell wall analysis-
dc.typeCapítulo de libro-
dc.identifier.doi10.1007/978-1-4614-2356-0_12-
dc.date.updated2015-06-09T11:49:13Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderJunta de Castilla y León-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
Appears in Collections:(IBFG) Libros y partes de libros
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show simple item record
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.