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Title

Fungal cell wall analysis

AuthorsPérez, Pilar; Ribas, Juan Carlos
KeywordsGlucan
Polysaccharides
Chitin
Mannan
Antifungal drugs
Cell wall
Issue Date2013
PublisherSpringer
CitationLaboratory Protocols in Fungal Biology: Current Methods in Fungal Biology: 175-196 (2013)
AbstractFungal cell wall is a rigid structure mainly composed of polysaccharides (up to 90 %) and glycoproteins. It is essential for survival of the fungal cells, because it protects them against bursting caused by internal turgor pressure and against mechanical injury. Because of its absence in mammalian cells, it is an attractive target for antifungal agents. Thus, for various reasons, it might be important to know how the cell wall is synthesized, and how to analyze its composition. We provide here information about in vitro analysis of the biosynthetic activities of the main fungal wall and describe some methods for rapid analysis of cell wall composition by using specific enzymatic degradations. We also describe some additional methods that can be occasionally used to analyze fungal wall properties or composition. These methods provide powerful tools to evaluate changes in fungal cell walls and will be useful for screening new compounds for antifungal activity that might cause inhibition of cell wall biosynthesis and/or alter the structure of the fungal cell wall.
URIhttp://hdl.handle.net/10261/116317
DOI10.1007/978-1-4614-2356-0_12
Identifiersdoi: 10.1007/978-1-4614-2356-0_12
isbn: 978-1-4614-2355-3
Appears in Collections:(IBFG) Libros y partes de libros
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