Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/116175
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Title

Mimicking insect communication: Release and detection of pheromone, biosynthesized by an alcohol acetyl transferase immobilized in a microreactor

AuthorsGuerrero, Muñoz, Lourdes Angel; Carot-Sans, Bula, Wojciech P. Gerard; Gardeniers, Han J. G. E.
KeywordsEnzymatic microreactor
insect chemistry
electroantennography
Spodoptera littoralis
pheromone
infochemical production
Issue Date4-Jun-2015
AbstractInfochemical production, release and detection of (Z,E)-9,11-tetradecadienyl acetate, the major component of the pheromone of the moth Spodoptera littoralis is achieved in a novel microfluidic system, designed to mimic the final step of the pheromone biosynthesis by immobilized recombinant alcohol acetyl transferase. The microfluidic system is part of an "artificial gland", i.e. a chemoemitter that comprises a microreactor connected to a microevaporator and is able to produce and release a pre-defined amount of the major component of the pheromone from the corresponding (Z,E)-9,11-tetradecadienol.. Performance of the entire chemoemitter has been assessed in electrophysiological and behavioral experiments. Electroantennographic depolarizations of the pheromone produced by the chemoemitter were ca. 40% relative to that evoked by the synthetic pheromone. In a wind tunnel, the pheromone released from the evaporator elicited on males a similar attraction behaviour as 3 virgin females in most of the parameters considered.
Publisher version (URL)http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0047751
URIhttp://hdl.handle.net/10261/116175
Appears in Collections:(CID) Artículos

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