Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/116088
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The Npl3 hnRNP prevents R-loop-mediated transcription-replication conflicts and genome instability |
Autor: | Santos-Pereira, José M. CSIC ORCID; Herrero, Ana B. CSIC ORCID; García-Rubio, María L. CSIC ORCID; Marín, Antonio; Moreno, Sergio CSIC ORCID; Aguilera, Andrés CSIC ORCID | Palabras clave: | R loops Npl3 hnRNPs |
Fecha de publicación: | 2013 | Editor: | Cold Spring Harbor Laboratory Press | Citación: | Genes and Development 27(22): 2445-2458 (2013) | Resumen: | Transcription is a major obstacle for replication fork (RF) progression and a cause of genome instability. Part of this instability is mediated by cotranscriptional R loops, which are believed to increase by suboptimal assembly of the nascent messenger ribonucleoprotein particle (mRNP). However, no clear evidence exists that heterogeneous nuclear RNPs (hnRNPs), the basic mRNP components, prevent R-loop stabilization. Here we show that yeast Npl3, the most abundant RNA-binding hnRNP, prevents R-loop-mediated genome instability. npl3¿ cells show transcription-dependent and R-loop-dependent hyperrecombination and genome-wide replication obstacles as determined by accumulation of the Rrm3 helicase. Such obstacles preferentially occur at long and highly expressed genes, to which Npl3 is preferentially bound in wild-type cells, and are reduced by RNase H1 overexpression. The resulting replication stress confers hypersensitivity to double-strand break-inducing agents. Therefore, our work demonstrates that mRNP factors are critical for genome integrity and opens the option of using them as therapeutic targets in anti-cancer treatment. | Descripción: | This article is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported). | Versión del editor: | http://dx.doi.org/10.1101/gad.229880.113 | URI: | http://hdl.handle.net/10261/116088 | DOI: | 10.1101/gad.229880.113 | ISSN: | 0890-9369 | E-ISSN: | 1549-5477 |
Aparece en las colecciones: | (IBMCC) Artículos (CABIMER) Artículos (IBFG) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Npl3 hnRNP.pdf | 1,42 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
47
checked on 24-mar-2024
SCOPUSTM
Citations
63
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
62
checked on 24-feb-2024
Page view(s)
398
checked on 23-abr-2024
Download(s)
254
checked on 23-abr-2024