English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/115994
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Towards a more realistic picture of in situ biocide actions: Combining physiological and microscopy techniques

AuthorsSperanza, Mariela ; Wierzchos, Jacek ; Ríos, Asunción de los ; Pérez-Ortega, Sergio; Souza-Egipsy, Virginia ; Ascaso, Carmen
KeywordsBiocide
Biodeterioration
Chlorophyll a fluorescence
Fungi
Lichen
Microorganisms
Ultrastructure
EM
Issue Date2012
PublisherElsevier
CitationScience of the Total Environment 439: 114–122 (2012)
AbstractIn this study, we combined chlorophyll a fluorescence (ChlaF) measurements, using pulseamplitude-modulate (PAM) equipment, with scanning electron microscopy in backscattered electron mode (SEM-BSE) and transmission electron microscopy (TEM) images to evaluate the actions of Koretrel at lower concentrations on Verrucaria nigrescens colonising a dolostone. ChlaF measurements are good indicators of the damaging effects of biocides. However, these indicators only provide an incomplete view of the mechanism of biocides used to control biodeterioration agents. The death of the V. nigrescens photobiont at two biocide concentrations was revealed by PAM, SEM-BSE and TEM. Once Koretrel was applied, the Fv/Fm ratios markedly fell in the first few hours after the 1.5% treatment, and ratios for the 3% dilution remained close to zero throughout the study. The algal zone shows the plasmolysed appearance of the photobiont cells, and important aspects related to the action of the biocide on free and lichenised fungi were also detected using SEM-BSE. Many of the mycobiont cells had only their cell walls preserved; although, some fungal hyphae in lichen thalli and some microorganisms in endolithic clusters maintained lipid storage in their cytoplasm. These results indicated that the combination of physiological and microscopy techniques improves the assessment of biocide action in situ and this will help to optimize protocols in order to reduce the emission of these compounds to the environment.
Publisher version (URL)http://dx.doi.org/10.1016/j.scitotenv.2012.09.040
URIhttp://hdl.handle.net/10261/115994
DOI10.1016/j.scitotenv.2012.09.040
ISSN0048-9697
Appears in Collections:(MNCN) Artículos
Files in This Item:
File Description SizeFormat 
Sc Total Environment, 439, 114–122 POSTPRINT.pdf7,59 MBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.