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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Castillo Alonso, Ana María | - |
dc.contributor.author | Sánchez-Díaz, Rosa Angélica | - |
dc.contributor.author | Vallés Brau, María Pilar | - |
dc.date.accessioned | 2015-06-01T08:25:34Z | - |
dc.date.available | 2015-06-01T08:25:34Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Castillo AM, Sánchez-Díaz RA, Vallés MP. Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association. Frontiers in Plant Science 6: 402 (2015) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/115943 | - |
dc.description | 31 Pags.- 7 Figs.- 4 Tabls. © 2015 Castillo, Sánchez-Díaz and Vallés. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). | es_ES |
dc.description.abstract | Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on a medium-low responding cultivar. Ovary genotype and developmental stage significantly affected microspore embryogenesis. By he use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism. Mature ovaries from flowers containing microspores at a late binucleate stage raised the number of embryos and green plants by 25% and 46% as compared to immature ovaries (excised from flowers with microspores at a mid-late uninucleate stage). The highest numbers of embryos and green plants were produced when using mature Caramba ovaries. Ovaries from Galeón, Tigre and Kilopondio cultivars successfully induced microspore embryogenesis at the same rate as Caramba ovaries. Moreover, Tigre ovaries raised the percentage of spontaneous chromosome doubling up to 71%. Attempts were made to identify molecular mechanisms associated to the inductive effect of the ovaries on microspore embryogenesis. The genes TAA1b, FLA26 and WALI6 associated to wheat microspore embryogenesis, the CGL1 gene involved in glycan biosynthesis or degradation, and the FER gene involved in the ovary signalling process were expressed and/or induced at different rates during ovary culture. The expression pattern of FLA26 and FER could be related to the differences between genotypes and developmental stages in the inductive effect of the ovary. Our results open opportunities for new approaches to increase bread wheat doubled haploid production by anther culture, and to identify the functional components of the ovary inductive effect on microspore embryogenesis. | es_ES |
dc.description.sponsorship | RA Sánchez-Díaz was a recipient of a predoctoral fellowship, from the Junta de Ampliación de Estudios, Consejo Superior de Investigaciones Científicas (JAE-CSIC) of Spain. This work was supported by Projects AGL2010-17509 and AGL2013-46698-R Plan Nacional de Recursos y Tecnologías Agroalimentarias of Spain and Diputacion General de Aragón (Grupo A06). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | wheat | - |
dc.subject | Anther culture | - |
dc.subject | ovary co-culture | - |
dc.subject | ovary developmental stage | - |
dc.subject | ovary genotype | - |
dc.subject | fasciclin-like arabinogalactan | - |
dc.subject | FERONIA | - |
dc.title | Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.3389/fpls.2015.00402 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.3389/fpls.2015.00402 | es_ES |
dc.identifier.e-issn | 1664-462X | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.relation.csic | Sí | es_ES |
dc.identifier.pmid | 26150821 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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CastilloAM_FrontPlantSci-2_2015.pdf | 109,77 kB | Adobe PDF | Visualizar/Abrir |
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