English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/115705
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 20 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Enhancement of chemokine function as an immunomodulatory strategy employed by human herpesviruses

Autor Viejo-Borbolla, Abel; Martínez-Martín, Nadia; Nel, Hendrik J.; Rueda, Patricia; Martín, Rocío; Blanco, Soledad; Arenzana-Seisdedos, Fernando; Thelen, Marcus; Fallon, Padraic; Alcamí, Antonio
Fecha de publicación 2012
EditorPublic Library of Science
Citación PLoS Pathogens 8 (2): e1002497 (2012)
ResumenHerpes simplex virus (HSV) types 1 and 2 are highly prevalent human neurotropic pathogens that cause a variety of diseases, including lethal encephalitis. The relationship between HSV and the host immune system is one of the main determinants of the infection outcome. Chemokines play relevant roles in antiviral response and immunopathology, but the modulation of chemokine function by HSV is not well understood. We have addressed the modulation of chemokine function mediated by HSV. By using surface plasmon resonance and crosslinking assays we show that secreted glycoprotein G (SgG) from both HSV-1 and HSV-2 binds chemokines with high affinity. Chemokine binding activity was also observed in the supernatant of HSV-2 infected cells and in the plasma membrane of cells infected with HSV-1 wild type but not with a gG deficient HSV-1 mutant. Cell-binding and competition experiments indicate that the interaction takes place through the glycosaminoglycan-binding domain of the chemokine. The functional relevance of the interaction was determined both in vitro, by performing transwell assays, time-lapse microscopy, and signal transduction experiments; and in vivo, using the air pouch model of inflammation. Interestingly, and in contrast to what has been observed for previously described viral chemokine binding proteins, HSV SgGs do not inhibit chemokine function. On the contrary, HSV SgGs enhance chemotaxis both in vitro and in vivo through increasing directionality, potency and receptor signaling. This is the first report, to our knowledge, of a viral chemokine binding protein from a human pathogen that increases chemokine function and points towards a previously undescribed strategy of immune modulation mediated by viruses.
URI http://hdl.handle.net/10261/115705
DOI10.1371/journal.ppat.1002497
Identificadoresdoi: 10.1371/journal.ppat.1002497
issn: 1553-7366
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
A_Alcami_Enhacement.pdf1,47 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.