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Título

Determination of L-canavanine and other free amino acids in Vicia disperma (Fabaceae) seeds by precolumn derivatization using diethyl ethoxymethylenemalonate and reversed-phase high-performance liquid chromatography

Autor Megías, Cristina ; Cortés-Giraldo, Isabel ; Girón-Calle, Julio ; Vioque, Javier ; Alaíz Barragán, Manuel
Palabras clave l-canavanine
Free amino acids
Vicia disperma
Diethyl ethoxymethylenemalonate
l-α-amino-γ-(guanidinooxy)-n-butyric acid
HPLC
Fecha de publicación 2014
EditorElsevier
Citación Talanta 131: 95- 98 (2014)
ResumenA method for determination of the non-protein amino acid l-α-amino-γ-(guanidinooxy)-n-butyric acid (l-canavanine) and other free amino acids in Vicia disperma is presented. Seed extracts were derivatized by reaction with diethyl ethoxymethylenemalonate and analyzed by reverse-phase high-performance liquid chromatography. Calibration curves showed very good linearity of the response. The limit of detection and quantification were 0.15 and 0.50 μM, respectively. The method has a high intra- (RSD=0.35%) and inter-repeatability (RSD=2.86%), and a remarkable accuracy with a 99% recovery in spiked samples. The method is very easy to carry out and allows for ready analysis of large number of samples using very basic HPLC equipment because the derivatized samples are very stable and have very good chromatographic properties. © 2014 Elsevier B.V.
URI http://hdl.handle.net/10261/115691
DOI10.1016/j.talanta.2014.07.077
Identificadoresdoi: 10.1016/j.talanta.2014.07.077
issn: 0039-9140
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