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Title

The interference of pharmaceuticals with endogenous and xenobiotic metabolizing enzymes in carp liver: an in-vitro study

AuthorsThibaut, Rémi; Schnell, Sabine; Porte Visa, Cinta
KeywordsXenobiotic
In vitro
Liver
Enzyme
Drug
Interference
Issue Date7-Jul-2006
PublisherAmerican Chemical Society
CitationEnvironmental Science & Technology 40(16): 5154-5160 (2006)
AbstractThe interactions of fibrate (clofibrate, fenofibrate, bezafibrate, gemfibrozil), antiinflammatory (ibuprofen, diclofenac, naproxen, ketoprofen), and anti-depressive (fluoxetine, fluvoxamine, paroxetine) drugs with CYP catalyzed pathways (CYP1A, CYP3A-, CYP2K-, and CYP2M-like) and Phase II activities (UDP-glucuronosyltransferases and sulfotransferases), involved in both xenobiotic and endogenous metabolism in fish, were investigated in-vitro by incubating carp liver subcellular fractions in the presence of the substrate and the selected drug. Antidepressive drugs were strong inhibitors of CYP1A (92-94% inhibition), CYP3A-like (69-80% inhibition), and CYP2Klike (36-69% inhibition) catalyzed activities, while antiinflammatory drugs were potent CYP2M-like inhibitors (32-74% inhibition). Among the lipid regulators, gemfibrozil strongly inhibited CYP2M-catalyzed activity (91% inhibition) and other CYP isoforms (CYP1A and CYP3A-like). Additionally, glucuronidation of naphthol and testosterone were targeted by antiinflammatory drugs, and to a lesser extent, by fibrate drugs (48-78% inhibition). No significant alteration on sulfotransferase activities was observed, apart from a minor inhibitory effect of clofibrate, gemfibrozil, and fluoxetine on the sulfation of estradiol. Overall, gemfibrozil, diclofenac, and the three anti-depressive drugs appear to be the pharmaceuticals with the highest potential to interfere with fish metabolic systems.
Description7 pages, 2 figures.-- PMID: 16955921 [PubMed].-- Printed version published Aug 15, 2006.
Publisher version (URL)http://dx.doi.org/10.1021/es0607483
URIhttp://hdl.handle.net/10261/11569
DOI10.1021/es0607483
ISSN0013-936x
E-ISSN1520-5851
Appears in Collections:(IDAEA) Artículos
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