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dc.contributor.authorRodriguez-Rodriguez, Patricia-
dc.contributor.authorFernández, Emilio-
dc.contributor.authorBolaños, Juan P.-
dc.date.accessioned2015-05-21T13:17:30Z-
dc.date.available2015-05-21T13:17:30Z-
dc.date.issued2013-
dc.identifierdoi: 10.1038/jcbfm.2013.168-
dc.identifierissn: 0271-678X-
dc.identifiere-issn: 1559-7016-
dc.identifier.citationJournal of Cerebral Blood Flow and Metabolism 33(12): 1843-1845 (2013)-
dc.identifier.urihttp://hdl.handle.net/10261/115600-
dc.description.abstractThe rates of glucose oxidized at glycolysis and pentose-phosphate pathway (PPP) in neurons are controversial. Using [3- 3 H]-, [1- 14 C]-, and [6- 14 C]glucose to estimate fluxes through these pathways in resting, intact rat cortical primary neurons, we found that the rate of glucose oxidized through PPP was, apparently, ∼14% of total glucose metabolized. However, inhibition of PPP rate-limiting step, glucose-6-phosphate (G6P) dehydrogenase, increased approximately twofold the glycolytic rate; and, knockdown of phosphoglucose isomerase increased ∼1.8-fold the PPP rate. Thus, in neurons, a considerable fraction of fructose-6-phosphate returning from the PPP contributes to the G6P pool that re-enters PPP, largely underestimating its flux. © 2013 ISCBFM.-
dc.description.sponsorshipJPB is funded by Spanish Ministerio de Economia y Competitividad (grant numbers SAF2010-20008 and RETICEF-RD12/0043/0021), European Regional Development Fund, and Junta de Castilla y Leon (SA003U13).-
dc.publisherNature Publishing Group-
dc.rightsclosedAccess-
dc.subjectAstrocytes-
dc.subjectNeuronal–glial interaction-
dc.subjectGlucose-
dc.subjectNeurochemistry-
dc.subjectEnergy metabolism-
dc.titleUnderestimation of the pentose-phosphate pathway in intact primary neurons as revealed by metabolic flux analysis-
dc.typeartículo-
dc.identifier.doi10.1038/jcbfm.2013.168-
dc.date.updated2015-05-21T13:17:30Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderEuropean Commission-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderJunta de Castilla y León-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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