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Underestimation of the pentose-phosphate pathway in intact primary neurons as revealed by metabolic flux analysis

AuthorsRodriguez-Rodriguez, Patricia ; Fernández, Emilio; Bolaños, Juan P.
Neuronal–glial interaction
Energy metabolism
Issue Date2013
PublisherNature Publishing Group
CitationJournal of Cerebral Blood Flow and Metabolism 33(12): 1843-1845 (2013)
AbstractThe rates of glucose oxidized at glycolysis and pentose-phosphate pathway (PPP) in neurons are controversial. Using [3- 3 H]-, [1- 14 C]-, and [6- 14 C]glucose to estimate fluxes through these pathways in resting, intact rat cortical primary neurons, we found that the rate of glucose oxidized through PPP was, apparently, ∼14% of total glucose metabolized. However, inhibition of PPP rate-limiting step, glucose-6-phosphate (G6P) dehydrogenase, increased approximately twofold the glycolytic rate; and, knockdown of phosphoglucose isomerase increased ∼1.8-fold the PPP rate. Thus, in neurons, a considerable fraction of fructose-6-phosphate returning from the PPP contributes to the G6P pool that re-enters PPP, largely underestimating its flux. © 2013 ISCBFM.
Identifiersdoi: 10.1038/jcbfm.2013.168
issn: 0271-678X
e-issn: 1559-7016
Appears in Collections:(IBFG) Artículos
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