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dc.contributor.authorSchultheiss, Kira P.-
dc.contributor.authorCraddock, Barbara P.-
dc.contributor.authorSuga, Hiroshi-
dc.contributor.authorMiller, W. Todd-
dc.identifierdoi: 10.1021/bi4016499-
dc.identifierissn: 0006-2960-
dc.identifiere-issn: 1520-4995-
dc.identifier.citationBiochemistry 53(8): 1320-1329 (2014)-
dc.descriptionACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.-
dc.description.abstractThe development of the phosphotyrosine-based signaling system predated the evolution of multicellular animals. Single-celled choanoflagellates, the closest living relatives to metazoans, possess numerous tyrosine kinases, including Src family nonreceptor tyrosine kinases. Choanoflagellates also have Csk (C-terminal Src kinase), the enzyme that regulates Src in metazoans; however, choanoflagellate Csk kinases fail to repress the cognate Src. Here, we have cloned and characterized Src and Csk kinases from Ministeria vibrans, a filasterean (the sister group to metazoans and choanoflagellates). The two Src kinases (MvSrc1 and MvSrc2) are enzymatically active Src kinases, although they have low activity toward mammalian cellular proteins. Unexpectedly, MvSrc2 has significant Ser/Thr kinase activity. The Csk homologue (MvCsk) is enzymatically inactive and fails to repress MvSrc activity. We suggest that the low activity of MvCsk is due to sequences in the SH2-kinase interface, and we show that a point mutation in this region partially restores MvCsk activity. The inactivity of filasterean Csk kinases is consistent with a model in which the stringent regulation of Src family kinases arose more recently in evolution, after the split between choanoflagellates and multicellular animals. © 2014 American Chemical Society.-
dc.publisherAmerican Chemical Society-
dc.relation.isversionofPublisher's version-
dc.titleRegulation of Src and Csk nonreceptor tyrosine kinases in the filasterean Ministeria vibrans-
dc.description.versionPeer Reviewed-
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