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Regulation of atrophin by both strands of the mir-8 precursor

AuthorsRubio Martínez, Mercedes ; Montañez, Raúl ; Pérez, Lidia; Millán, Marco; Bellés, Xavier
miRNA passenger strand
Issue DateNov-2013
CitationInsect Biochemistry and Molecular Biology 43(11): 1009-1014 (2013)
AbstractIn Drosophila melanogaster, miR-8-3p regulates mRNA levels of atrophin, a factor involved in neuromotor coordination, and we found that Blattella germanica with suppressed atrophin showed motor problems. Bionformatic predictions and luciferase-reporter tests indicated that B.germanica atrophin mRNA contains target sites for miR-8-3p and miR-8-5p. Suppression of miR-8-3p or miR-8-5p appeared to increase atrophin mRNA. The effects of suppression of Argonaute (AGO) 1 or AGO2 expression on miR-8-3p and miR-8-5p suggested that miR-8-3-p might predominantly bind to AGO1, whereas miR-8-5p might bind to a moderate extent to both AGO1 and AGO2 in the respective RNA-induced silencing complexes (RISCs). We propose that the interplay of miR-8-3p, miR-8-5p, AGO1 and AGO2, maintain the appropriate levels of atrophin mRNA. This would be the first example of two strands of the same miRNA precursor regulating a single transcript. © 2013 Elsevier Ltd.
Publisher version (URL)http://dx.doi.org/10.1016/j.ibmb.2013.08.003
Identifiersdoi: 10.1016/j.ibmb.2013.08.003
issn: 0965-1748
Appears in Collections:(IBE) Artículos
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