English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/115387
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 25 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Stability of isolated bacterial and photosystem II reaction center complexes on Ag electrode surfaces. A surface-enhanced resonance Raman study

Autor Picorel Castaño, Rafael ; Holt, Randall E.; Heald, R.; Cotton, T. M.; Seibert, Michael
Fecha de publicación 1991
EditorAmerican Chemical Society
Citación Journal of the American Chemical Society 113: 2839- 2843 (1991)
ResumenSurface-enhanced resonance Raman scattering (SERRS) spectra from isolated Rhodospirillum rubrwn bacterial reaction center complexes indicate that the carotenoid, spirilloxanthin, resides in the cis conformation, while SERRS spectra from isolated photosystem II (PSII) D1-D2-cytochrome b-559 reaction center complexes show that β-carotene is in the all-trans conformation. The fact that the cis conformation of the native bacterial reaction center carotenoid is maintained during SERRS experiments suggests that significant denaturation of the protein matrix does not occur as a result of contact with the anodized Ag metallic surface required for observation of SERRS spectra. Although a similar conclusion cannot be drawn from observations of β-carotene in PSII reaction centers, no new spectral bands previously ascribed to denatured cytochrome (Cyt) heme groups are observed with Cyt b-559 during SERRS experiments. Specific enhancement of SERRS signals from Cyt b-559 compared to the other chromophores of the PSII reaction center and in particular observation of a strong 1360-cm-1 band indicate that the heme group is located on or very close to the surface of the PSII reaction center complex and can be reduced by the Ag electrode.
Versión del editorhttps://dx.doi.org/10.1021/ja00008a006
URI http://hdl.handle.net/10261/115387
DOI10.1021/ja00008a006
Identificadoresdoi: 10.1021/ja00008a006
issn: 0002-7863
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.