English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/115363
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 50 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Pigment stoichiometry of a newly isolated D1-D2-Cyt b559 complex from the higher plant Beta vulgaris L.

Autor Montoya Blanco, Guillermo ; Yruela Guerrero, Inmaculada ; Picorel Castaño, Rafael
Palabras clave Beta vulgaris
Stoichiometry
Pigment
Reaction center
Photosystem II
Fecha de publicación 1991
EditorElsevier
Citación FEBS Letters 283: 255- 258 (1991)
ResumenTwo D1-D2-Cyt b559 complexes with different pigment stoichiometry were isolated from the higher plant B. vulgaris. The procedures for isolating both complexes only differed in the washing time of the DEAE column with 50 mM Tris-HCl, pH 7.2, 0.05% Triton X-100 and 30 mM NaCl. When the column was washed until the eluate had an absorbance of 0.01 at 670 nm, the isolated D1-D2-Cyt b559 complex presented a pigment stoichiometry of 6 chlorophyll a, 2 β-carotene, and 1 cytochrome b559 per 2 pheophytin a. In contrast, when the column was exhaustively washed until the eluate reached an absorbance of 0.005 at 670 nm, the complex had a stoichiometry of 4 chlorophyll a, 1 β-carotene, and 1 cytochrome b559 per 2 pheophytin a. We think that the former stoichiometry corresponds to that of the native Da-D2-Cyt b559 complex. Moreover, both preparations showed 2 mol of pheophytin a per 1 mol of reaction center protein.
Versión del editorhttp://dx.doi.org/10.1016/0014-5793(91)80601-X
URI http://hdl.handle.net/10261/115363
DOI10.1016/0014-5793(91)80601-X
Identificadoresdoi: 10.1016/0014-5793(91)80601-X
issn: 0014-5793
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.