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dc.contributor.authorFayos Avellán, Oreto-
dc.contributor.authorVallés Brau, María Pilar-
dc.contributor.authorGarcés-Claver, Ana-
dc.contributor.authorMallor Giménez, Cristina-
dc.contributor.authorCastillo Alonso, Ana María-
dc.date.accessioned2015-05-19T07:05:25Z-
dc.date.available2015-05-19T07:05:25Z-
dc.date.issued2015-
dc.identifier.citationFayos O, Vallés MP, Garcés-Claver A, Mallor C, Castillo AM. Doubled haploid production from Spanish onion (Allium cepa L.) germplasm: embryogenesis induction, plant regeneration and chromosome doubling. Frontiers in Plant Science 6: 384 (2015)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/115359-
dc.description28 Pags.- 6 Tabls.- 1 Fig. This Document is Protected by copyright and was first published by Frontiers. All rights reserved. it is reproduced with permission (Creative Commons Attribution License).es_ES
dc.description.abstractThe use of doubled haploids in onion breeding is limited due to the low gynogenesis efficiency of this species. Gynogenesis capacity from Spanish germplasm, including the sweet cultivar Fuentes de Ebro, the highly pungent landrace BGHZ1354 and the two Valenciana type commercial varieties Recas and Rita, was evaluated and optimized in this study. The OH-1 population, characterized by a high gynogenesis induction, was used as control. Growing conditions of the donor plants were tested with a one-step protocol and field plants produced a slightly higher percentage of embryogenesis induction than growth chamber plants. A one-step protocol was compared with a twostep protocol for embryogenesis induction. Spanish germplasm produced a 2 to 3 times higher percentage of embryogenesis with the two-step protocol, Recas showing the highest percentage (2.09%) and Fuentes de Ebro the lowest (0.53%). These percentages were significantly lower than those from the OH-1 population, with an average of 15% independently of the protocol used. The effect of different containers on plant regeneration was tested using both protocols. The highest percentage of acclimated plants was obtained with the two-step protocol in combination with Eco2box (70%), whereas the lowest percentage was observed with glass tubes in the two protocols (20- 23%). Different amiprofos-methyl (APM) treatments were applied to embryos for chromosome doubling. A similar number of doubled haploid plants were recovered with 25 or 50 μM APM in liquid medium. However, the application of 25 μM in solid medium for 24 h produced the highest number of doubled haploid plants. Somatic regeneration from flower buds of haploid and mixoploid plants proved to be a successful approach for chromosome doubling, since diploid plants were obtained from the 4 regenerated lines. In this study, doubled haploid plants were produced from the four Spanish cultivars, however further improvements are needed to increase their gynogenesis efficiency.es_ES
dc.description.sponsorshipO. Fayos was a recipient of a predoctoral fellowship from the Spanish Ministry of Economy and Competitiveness FPI (INIA-FEDER). This work was supported by the Spanish Ministry of Economy and Competitiveness INIA-FEDER (RTA2011-00118- C02-01), by the Agreement EEAD-CSIC with CITA-Aragón for Onion Doubled Haploid Production and the Aragon Government (Group A16 and Group A06).es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectoniones_ES
dc.subjectgynogenesises_ES
dc.subjectSpanish germplasmes_ES
dc.subjectflower budes_ES
dc.subjectembryogenesises_ES
dc.subjectEco2boxes_ES
dc.subjectChromosome doublinges_ES
dc.titleDoubled haploid production from Spanish onion (Allium cepa L.) germplasm: embryogenesis induction, plant regeneration and chromosome doublinges_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.3389/fpls.2015.00384-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3389/fpls.2015.00384es_ES
dc.identifier.e-issn1664-462X-
dc.rights.licensehttp://creativecommons.org/licenses/by/3.0/es/es_ES
dc.relation.csices_ES
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