English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/115278
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)

Polygalacturonase, cellulase and invertase activities during cherimoya fruit ripening

Autor Sánchez, J. A.; Zamorano, J. P.; Alique, Rafael
Fecha de publicación 1998
EditorHeadley Bros
Citación Journal of Horticultural Science and Biotechnology 73: 87- 92 (1998)
ResumenThe activities of the cell-wall degrading enzymes polygalacturonase (PG) and cellulase and of two forms of invertase were studied during the ripening of cherimoya fruit. All activities showed maxima coinciding with the climacteric maximum in respiration and the beginning of the most pronounced rise in ethylene production. The activities of PG and cellulase increased three-fold concomitantly with the highest drop in flesh firmness. After reaching maxima, the activity of the soluble invertase was almost stabilized, while that of the cell-wall bound form decreased to values similar to those of preclimacteric fruit. Since fructose and glucose concentrations increased continuously during ripening it is suggested that sucrose was hydrolyzed mainly by the soluble invertase. Cherimoya PG and cellulase had maximum activities at pH 5.0 and 5.5, respectively. PG activity was more thermostable than cellulase activity (complete inactivation by heating for 5 min at 75°C and 60°C, respectively). The activity of both invertases had a similar pH optimum (3.0-3.5), heat stability (complete inactivation by heating for 5 min at 55-60°C) and affinity for sucrose (K(m) of 2.6 and 3.8 mM).
URI http://hdl.handle.net/10261/115278
Identificadoresissn: 1462-0316
Aparece en las colecciones: (IF) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.