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Changes in the composition and activity of a complex microbiota developed in a Simulator of the Human Intestinal Microbial Ecosystem after addition of Lactobacillus plantarum IFPL935

AutorBarroso, Elvira ; Martínez-Cuesta, M. Carmen ; Wiele, Tom van de; Requena, Teresa ; Peláez, Carmen
Fecha de publicación2014
CitaciónTargeting Microbiota (2014)
ResumenPrevious studies have demonstrated that the potential probiotic Lactobacillus plantarum IFPL935 strain is able to grow in the presence of a flavan-3-ol extract from grape seeds and also to initiate the metabolism of these polyphenols to other compounds. This study aims to investigate the role of L. plantarum IFPL935 on the composition and/or activity of a human intestinal microbiota in a long-term feeding assay with a polyphenolic red wine extract. For that purpose, after a 3-weeks stabilization period of the colonic microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (Twin-SHIME), a 2-weeks experiment was run by feeding both parallel systems daily with 200 mg of a red wine extract. In addition, one of the Twin-SHIME systems was simultaneously fed with L. plantarum IFPL935 (1010 cfu, daily). Microbial community changes along with fermentative and proteolytic activities were monitored. The results showed that feeding the gut microbiota with the phenolic extract slightly decreased total bacterial counts in the ascendens (AC) reactor (1 log) after 24 h. All bacterial groups analyzed were affected. Among them, Bacteroides, Bifidobacterium and the butyrate producers Clostridium cocoides/Eubacterium rectale were the most affected groups. In most cases, bacterial numbers were readily recovered after the first week of treatment. Moreover, addition of L. plantarum IFPL935 to one of the Twin-SHIME systems resulted in relative stable numbers of total bacteria, Lactobacillus and Enterobacteriaceae. Related to butyrate producers, BCoAT gene copy numbers showed significantly higher values in the transverse (TC) and descendens (DC) reactors than in the AC ones at the end of the treatment, and particularly, in the DC reactor corresponding to the SHIME unit supplemented with L. plantarum IFPL935. Regarding fermentation and proteolysis markers, daily intake of the polyphenolic extract caused a sharp decrease during the first days of treatment in all the colon reactors. Addition of L. plantarum IFPL935 led to an increase of lactic acid in the ascendens colon compartment (AC) at the starting of the period studied and of a butyric acid in the distal colon vessels at the end of the treatment (TC and DC). No differences in proteolysis markers between treatments were observed when L. plantarum IFPL935 was added. The SHIME supplementation with L. plantarum IFPL935 resulted in relatively stable numbers of total bacteria, lactobacilli and enterobacteria. Besides, addition of L. plantarum IFPL935 may possibly influence SCFAs production, and in particular butyrate, which have numerous documented effects promoting bowel large function.
DescripciónResumen del póster presentado al 2nd World Congress on Targeting Microbiota celebrado en el Institut Pasteur de Paris del 16 al 17 de octubre de 2014.
Aparece en las colecciones: (CIAL) Comunicaciones congresos
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