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Lactobacillus plantarum IFPL935 improves colonic metabolism in a simulator of the human intestinal microbial ecosystem during feeding with red wine phenolic compounds

AutorBarroso, Elvira ; Wiele, Tom van de; Jiménez-Girón, Ana ; Muñoz-González, Irene ; Moreno-Arribas, M. Victoria ; Bartolomé, Begoña ; Bartolomé, Begoña ; Peláez, Carmen ; Martínez-Cuesta, M. Carmen ; Requena, Teresa
Fecha de publicación2013
Citación2nd ICFD (2013)
ResumenWe have evaluated the adaptation of the colonic microbiota developed in two parallel five-stage in vitro gastrointestinal simulators (TWINSHIME) to long-term feeding with a polyphenol-rich red wine extract. Microbial evolution and metabolism were compared with simultaneous feeding of one of the SHIME models with a daily intake of 10 log cfu of Lactobacillus plantarum IFPL935. The onset of the polyphenolic extract addition caused a sharp reduction of the metabolic activity (short-chain fatty acids and ammonium production) in the three colon vessels ascendens (CA), transversum (CT) and descendens (CD). The metabolic reduction was most apparent in vessel CA, which also showed a reduction of total bacteria in 1 log genomic copies during the first day of treatment. When the SHIME was fed with L. plantarum IFPL935, a significant increase of butyrate production was observed during the phenolic treatment and the washout period in vessels CT and CD compared with the vessels without the strain. The increase of butyrate was associated with a faster recovery of the butyrate-producing bacteria Clostridium leptum, Clostridium coccoides-Eubacterium rectale group and Ruminococcus in the SHIME fed with L. plantarum IFPL935. Microbial catabolism of wine polyphenols was also monitored by targeted analysis of phenolic acids and other intermediate metabolites. After feeding the TWINSHIME with the polyphenol-rich red wine extract, it was observed the production of numerous phenolic metabolites, mainly phenolic acids, coming from both anthocyanidin and non-anthocyanidin compounds, starting after 4-5 days of treatment. Phenolic catabolism varied depending on the colonic vessels, the changes in microbial population and the presence of L. plantarum IFPL935. Overall, L. plantarum IFPL935 was able to improve colonic metabolism by promoting the formation of biologically significant compounds.
DescripciónTrabajo presentado al 2nd International Conference on Food Digestion celebrado en Madrid (España) del 6 al 8 de marzo de 2013.
Versión del editorhttps://colloque6.inra.fr/cost_infogest_icfd2013
Aparece en las colecciones: (CIAL) Comunicaciones congresos
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