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Partial characterization of polyphenol oxidase activity in raspberry fruits

AuthorsGonzález, E. M.; Ancos, Begoña de ; Cano, M. Pilar
Polyphenol oxidase
Issue Date1999
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 47: 4068- 4072 (1999)
AbstractA partial characterization of polyphenol oxidase (PPO) activity in raspberry fruits is described. Two early cultivars harvested in May/June (Heritage and Autumm Bliss) and two late cultivars harvested in October- November (Ceva and Rubi) were analyzed for PPO activity. Stable and highly active PPO extracts were obtained using insoluble poly(vinylpyrrolidone) (PVP) and Triton X-100 in sodium phosphate, pH 7.0 buffer. Polyacrylamide gel electrophoresis of raspberry extracts under nondenaturing conditions resolved in one band (R(fl) = 0.25). Raspberry PPO activity has pH optima of 8.0 and 5.5, both with catechol (0.1 M). Maximum activity was with D-catechin (catecholase activity). followed by p-coumaric acid (cresolase activity). Heritage raspberry also showed PPO activity toward 4-methylcatechol. Ceva and Autumm Bliss raspberries showed the higher PPO activity using catechol as substrate.
Identifiersdoi: 10.1021/jf981325q
issn: 0021-8561
Appears in Collections:(IF) Artículos
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