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Título

Partial characterization of polyphenol oxidase activity in raspberry fruits

Autor González, E. M.; Ancos, Begoña de ; Cano, M. Pilar
Palabras clave Native-PAGE
Polyphenol oxidase
Characterization
Raspberry
Fecha de publicación 1999
EditorAmerican Chemical Society
Citación Journal of Agricultural and Food Chemistry 47: 4068- 4072 (1999)
ResumenA partial characterization of polyphenol oxidase (PPO) activity in raspberry fruits is described. Two early cultivars harvested in May/June (Heritage and Autumm Bliss) and two late cultivars harvested in October- November (Ceva and Rubi) were analyzed for PPO activity. Stable and highly active PPO extracts were obtained using insoluble poly(vinylpyrrolidone) (PVP) and Triton X-100 in sodium phosphate, pH 7.0 buffer. Polyacrylamide gel electrophoresis of raspberry extracts under nondenaturing conditions resolved in one band (R(fl) = 0.25). Raspberry PPO activity has pH optima of 8.0 and 5.5, both with catechol (0.1 M). Maximum activity was with D-catechin (catecholase activity). followed by p-coumaric acid (cresolase activity). Heritage raspberry also showed PPO activity toward 4-methylcatechol. Ceva and Autumm Bliss raspberries showed the higher PPO activity using catechol as substrate.
URI http://hdl.handle.net/10261/114847
DOI10.1021/jf981325q
Identificadoresdoi: 10.1021/jf981325q
issn: 0021-8561
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