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dc.contributor.authorHermida, Carmen-
dc.contributor.authorMartínez-Costa, Oscar H.-
dc.contributor.authorCorrales, Guillermo-
dc.contributor.authorSánchez, Valentina-
dc.contributor.authorCodoceo, Rosa-
dc.contributor.authorFernández-Mayoralas, Alfonso-
dc.contributor.authorAragón, Juan J.-
dc.date.accessioned2015-05-07T09:32:08Z-
dc.date.available2015-05-07T09:32:08Z-
dc.date.issued2014-
dc.identifierdoi: 10.1002/jcla.21713-
dc.identifierissn: 0887-8013-
dc.identifiere-issn: 1098-2825-
dc.identifier.citationJournal of Clinical Laboratory Analysis 28(6): 478-486 (2014)-
dc.identifier.urihttp://hdl.handle.net/10261/114829-
dc.descriptionet al.-
dc.description.abstract[Background]: The phloroglucinol assay is the current method for d-xylose determination in urine/plasma/serum. However, its sensitivity is limited when low amounts of d-xylose are to be measured, such as in the noninvasive evaluation of intestinal lactase with 4-galactosylxylose (gaxilose). An improved assay was therefore needed. [Methods]: We developed and validated a modified version of the phloroglucinol-based assay for quantification of d-xylose in urine/serum samples. A method for gaxilose determination by gas chromatography (GC) was also optimized. [Results]: Linearity ranged from 0.125 to 5.0 mg/l (5¿200 mg/l in original sample). Accuracy at LOQ (0.125 mg/l) was 0.97/2.49% in spiked urine/serum; for other quality controls (QC), it was <1.27%. Intra- and interassay precision at LOQ were 6.02% and 6.45% for urine, and 8.86% and 10.00%, respectively, for serum; for other QC, precision was <2.15%. Linearity of gaxilose determination by GC was 3.90¿195.17 for urine and 9.75-195.17 mg/l for serum with acceptable sensitivity and reproducibility. The method proved adequate for the d-xylose determination in healthy and hypolactasic subjects after oral administration of gaxilose. [Conclusions]: The modified method provides high sensitivity and robustness for d-xylose quantification in urine/serum for routine clinical use especially in the noninvasive diagnosis of intestinal lactase deficiency with the gaxilose test.-
dc.description.sponsorshipGrant sponsor: Ministerio de Ciencia e Innovación; Grant number: BFU2009–13114; Grant sponsor: Comunidad de Madrid; Grant number: S2009/PPQ-1752; Grant sponsor: Venter Pharma SL.-
dc.publisherJohn Wiley & Sons-
dc.relationS2009/PPQ-1752/CAPOTE-
dc.rightsclosedAccess-
dc.subjectGaxilose-
dc.subjectHypolactasia-
dc.subjectIntestinal lactase-
dc.subject4-galactosylxylose-
dc.subjectphloroglucinol-
dc.subjectD-xylose-
dc.subjectAnalytical validation-
dc.titleImprovement and Validation of d-Xylose Determination in Urine and Serum as a New Tool for the Noninvasive Evaluation of Lactase Activity in Humans-
dc.typeartículo-
dc.identifier.doihttp://dx.doi.org/10.1002/jcla.21713-
dc.date.updated2015-05-07T09:32:08Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderComunidad de Madrid-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderVenterPharma-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
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