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dc.contributor.authorClemente, Alfonso-
dc.contributor.authorMarín-Manzano, M. Carmen-
dc.contributor.authorRubio, Luis A.-
dc.contributor.authorHernández-Hernández, Oswaldo-
dc.contributor.authorSanz Herranz, Yolanda-
dc.contributor.authorLaparra, José Moisés-
dc.contributor.authorSanz, M. Luz-
dc.contributor.authorMontilla, Antonia-
dc.contributor.authorOlano, Agustín-
dc.contributor.authorMoreno, F. Javier-
dc.date.accessioned2015-05-06T13:21:12Z-
dc.date.available2015-05-06T13:21:12Z-
dc.date.issued2012-
dc.identifier.citationSOMED 2012-
dc.identifier.urihttp://hdl.handle.net/10261/114801-
dc.descriptionResumen del póster presentado al 35th International Congress of the Society for Microbial Ecology and Disease (SOMED) celebrado en VAlencia (España) del 15 al 17 de mayo de 2012.-
dc.description.abstract[Introduction]: Galacto-oligosaccharides are considered functional dietary compounds capable of modulating the composition and metabolic activities of intestinal microbiota. Indeed, lactulose-derived galacto-oligosaccharides are attracting increasing attention due to their prospective prebiotic applications. [Objectives]: The aims of this study are to evaluate a) the resistance to digestion of novel lactulosederived galacto-oligosaccharides and b) if their major components are fermented by the microbiota to promote the selective growth of beneficial bacteria in the large intestine. [Method/Design]: Lactulose-derived galacto-oligosaccharides were incorporated in a single dose (1%, w:w) to rats for a period of fourteen days. Chromium oxide was included in diets as an indigestible marker. Fecal samples were collected weekly whereas the intestinal contents of rats were collected at the end of the dietary intervention period. Di- and trisaccharides were identified and quantified in dietary, ileum and fecal samples by GCMS. Different microbial groups were quantified using qPCR, and the Bifidobacteria group analysed by PCR-denaturing gradient gel electrophoresis. [Results]: Quantitative evaluation of carbohydrates from dietary and ileal samples demonstrated that the disaccharide fraction of lactulose-derived galacto-oligosaccharides, mostly composed of β-galactobioses and galactosyl-fructoses, was fully resistant to digestion in the small intestine. The trisaccharide fraction exhibited a limited digestion as evaluated in ileal samples of treated animals. The chromatographic profile of fecal samples of rats fed lactulose-derived galacto-oligosaccharides demonstrated the complete fermentation of galacto-oligosaccharides in the large intestine. A significant increase in the growth of both Bifidobacteria and Eubacterium rectale/Clostridium coccoides groups was observed by qPCR in response to the dietary treatment in large intestine; at specie level, a significant and selective increase of Bifidobacterium animalis was revealed. [Conclusions]: The reported data demonstrate the resistance to digestion and fermentation selectivity of lactulose-derived galacto-oligosaccharides by specific bifidobacteria species and support their potential role as prebiotic ingredient in functional foods.-
dc.rightsclosedAccess-
dc.titleResistance to mammalian digestion and prebiotic properties of novel galacto-oligosaccharides from lactulose-
dc.typepóster de congreso-
dc.date.updated2015-05-06T13:21:13Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.relation.csic-
dc.type.coarhttp://purl.org/coar/resource_type/c_6670es_ES
item.openairetypepóster de congreso-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
Aparece en las colecciones: (EEZ) Comunicaciones congresos
(IQOG) Comunicaciones congresos
(IATA) Comunicaciones congresos
(CIAL) Comunicaciones congresos
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