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Screening of food-derived opioid peptides on mucin production by human intestinal cells

AutorMartínez-Maqueda, D. ; Miralles, Beatriz ; Pascual-Teresa, Sonia de ; Reverón, Inés ; Muñoz, Rosario ; Recio, Isidra
Fecha de publicación2012
Citación3rd MC & WG Meeting (2012)
ResumenThe in vivo release of active peptides during food digestion is well-established. Some milk-derived peptides with opioid activity, named β-casomorphins, have been found in the intestinal lumen of the neonate and the adult milk consumer. Although their physiological relevance is still unknown, a functional role as opioid receptor ligands in the gastrointestinal tract is likely (Teschemacher et al., 1997). It has been demonstrated that β-casomorphin 7 stimulates the production of mucin via a direct action on epithelial goblet cells using HT29-MTX cells as a model of human colonic epithelium (Zoghbi et al., 2006). In this study, we have investigated if other opioid peptides that can be originated from milk or wheat digestion might increase the secretion or gene expression of mucin in this cell model. Eight milk peptides and a wheat gluten-derived peptide with proved or probable ability to bind µ- or δ-opioid receptors were tested. Six peptides, besides the previously described β-casomorphin 7, increased significantly the secretion of mucins, as measured by an enzyme-linked lectin assay (ELLA). Human β-casomorphin 5 and α-lactorphin were selected to study the expression of the HT29-MTX major secreted mucin gene (MUC5AC). α-Lactorphin showed increased expression of MUC5AC from 4 to 24 h, up to 1.6-fold over basal level expression. However, this increased expression of MUC5AC did not reach significance after cell treatment with human β-casomorphin 5. In conclusion, six food-derived peptides with reported or probable opioid activity have been first found to induce mucin secretion in HT29-MTX cells. Concretely, α-lactorphin increased the expression of the major secreted mucin gene encoded by these cells.
DescripciónResumen del póster presentado al 3rd Management Committee & Working Groups Meeting of COST Action FA 1005 celebrado en Leatherhead (UK) del 2 al 4 de octubre de 2012.
Aparece en las colecciones: (ICTAN) Comunicaciones congresos
(CIAL) Comunicaciones congresos
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