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dc.contributor.authorJaumot, Joaquim-
dc.contributor.authorEritja Casadellà, Ramón-
dc.contributor.authorTauler, Romà-
dc.contributor.authorGargallo, Raimundo-
dc.identifier.citationNucleic Acids Research 34(1): 206-216 (2006)en_US
dc.description11 pages, 5 figures, 2 schemes.-- PMID: 16397299 [PubMed].en_US
dc.description.abstractThe resolution of the dimeric intermolecular G-quadruplex/duplex competition of the telomeric DNA sequence 5'-TAG GGT TAG GGT-3' and of its complementary 5' ACC CTA ACC CTA-3' is reported. To achieve this goal, melting experiments of both sequences and of the mixtures of these sequences were monitored by molecular absorption, molecular fluorescence and circular dichroism spectroscopies. Molecular fluorescence measurements were carried out using molecular beacons technology, in which the 5'-TAG GGT TAG GGT-3' sequence was labelled with a fluorophore and a quencher at the ends of the strand. Mathematical analysis of experimental spectroscopic data was performed by means of multivariate curve resolution, allowing the calculation of concentration profiles and pure spectra of all resolved structures (dimeric antiparallel and parallel G-quadruplexes, Watson–Crick duplex and single strands) present in solution. Our results show that parallel G-quadruplex is more stable than antiparallel G-quadruplex. When the complementary C-rich strand is present, a mixture of both G-quadruplex structures and Watson–Crick duplex is observed, the duplex being the major species. In addition to melting temperatures, equilibrium constants for the parallel/antiparallel G-quadruplex equilibrium and for the G-quadruplex/duplex equilibrium were determined from the concentration profiles.en_US
dc.description.sponsorshipThanks are due to Dr Shantanu Chowdhury (IGIB, Delhi, India) for his helpful comments. J.J. acknowledges a PhD scholarship from the Spanish Ministerio de Educación y Ciencia (MEC). This research was supported by the grants BQU2003-0191 and BFU2004-02048/BMC. Funding to pay the Open Access publication charges for this article was provided by Spanish Ministerio de Educacion y Ciencia.en_US
dc.format.extent162 bytes-
dc.publisherOxford University Pressen_US
dc.subjectStructural competitionen_US
dc.subjectDimeric paralell/antiparalell G-quadruplexen_US
dc.subjectC-rich complementary stranden_US
dc.subjectMixture sequencesen_US
dc.titleResolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary stranden_US
dc.description.peerreviewedPeer revieweden_US
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