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Characterization of galactooligosaccharides by HILIC-MSn and GC-MS

AutorHernández-Hernández, Oswaldo ; Lebrón-Aguilar, Rosa ; Moreno, F. Javier ; Sanz, M. Luz
Fecha de publicación2012
CitaciónWorkshop Carbohydrate analysis and glycomics: where next? (2012)
ResumenGalactooligosaccharides (GOS) are complex mixtures of prebiotic carbohydrates obtained by transgalactosylation reactions of lactose, and more recently lactulose, by b-galactosidases of different origins. Different structures are obtained depending on the enzymatic source, temperature of synthesis, etc. Considering the substantial influence of the chemical structure on the prebiotic activity of oligosaccharides, the characterization of these compounds is of vital importance for the elucidation of the structure-function relationship. In this work, GOS obtained from both lactose and lactulose were characterized following two different approaches: (i) the development of a new method using Hydrophilic Interaction Liquid Chromatography coupled to tandem Mass Spectrometry (HILIC-MSn). Three different stationary phases (sulfoalkylbetaine zwitterionic, polyhydroxyethyl aspartamide and ethylene bridge hybrid (BEH) with trifunctionally-bonded amide), were assayed and compared. The best results were achieved using the BEH column, showing resolution values higher than 1 for most carbohydrates. Characteristic MS2 fragmentation profiles of disaccharides containing galactose units with different linkages were evaluated and used for the characterization of di-, tri- and tetrasaccharides of different prebiotic GOS mixtures. (ii) the use of Gas Chromatography coupled to Mass Spectrometry (GC-MS) of the trimethylsilyl oximes of GOS. Main carbohydrates of these mixtures (di- and trisaccharides) were analysed establishing a relationship between their m/z characteristic ionization profile and their chemical structure. Monosaccharide moieties and glycosidic linkages were tentatively assigned by this method. Both chromatographic techniques offer complementary information for the characterization of GOS.
DescripciónResumen del póster presentado al Workshop Carbohydrate analysis and glycomics: where next? celebrado en Londres (UK) el 21 de marzo de 2012.
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