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Título

Induction of CYP1A by the N-imidazole derivative, 1-benzylimidazole

AutorNavas, José M.; Chana López, Antonio ; Herradón García, Bernardo ; Segner, Helmut
Palabras claveCytochrome P4501A
Imidazole
Aryl hydrocarbon receptor
Rainbow trout
Hepatocyte
Fecha de publicaciónabr-2003
EditorSETAC (Society)
CitaciónEnvironmental Toxicology and Chemistry 22(4): 830-836 (2003)
ResumenXenobiotics can induce cytochrome P4501A (CYP1A) by ligand binding to the aryl hydrocarbon receptor (AhR). Typical AhR ligands are polycyclic aromatic compounds with planar molecular conformation. The present work investigated the ability of the N-imidazole derivative, 1-benzylimidazole (BIM), to induce CYP1A in rainbow trout hepatocytes. Benzylimidazole increased hepatocellular CYP1A catalytic activity (determined as 7-ethoxyresorufin-O-deethylase [EROD] activity) and CYP1A mRNA in a concentration-dependent way. Computational studies on the molecular structure of BIM indicated that the energetically most stable BIM conformer has the imidazole ring and the phenyl ring in different planes, i.e., does not take a planar conformation. This property of BIM does not agree with the structural requirements of a typical AhR ligand. In line with this observation, we found that the AhR antagonist, α-naphthoflavone (αNF), was not able to inhibit BIM induction of EROD activity and CYP1A mRNA, although it inhibited the induction of CYP1A by the prototypic AhR ligand, β-naphthoflavone (βNF). The results suggest that transcriptional activation of CYP1A by the N-imidazole derivative, BIM, is not mediated through direct ligand binding to the AhR.
Descripción7 pages.-- PMID: 12685719 [PubMed].
Versión del editorhttp://dx.doi.org/10.1897/1551-5028(2003)022<0830:IOCBTN>2.0.CO;2
URIhttp://hdl.handle.net/10261/11458
DOI10.1897/1551-5028(2003)022<0830:IOCBTN>2.0.CO;2
ISSN0730-7268 (Print)
1552-8618 (Online)
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