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Hydrolysis of the milk allergen β-lactoglobulin using high hydrostatic pressure abrogates allergenicity in vivo

AuthorsLópez-Expósito, Iván ; Chicón, Rosa; Belloque, Josefina ; López-Fandiño, Rosina ; Berin, M. Cecilia
Issue Date2011
CitationAAAAI Annual Meeting 2011
Abstract[Rationale]: The major milk allergen b-Lactoglobulin (b-Lg) exhibits enhanced susceptibility to proteolysis under high hydrostatic pressure and this may be an efficient method to produce hypoallergenic hydrolysates. The aimof thisworkwas to evaluate the allergenicity of 3 b-Lg hydrolysates. [Methods]: Hydrolysates were prepared as follows: A: Pepsin treated, 5 min, 400 mPa pressure; B: Chymotrypsin, 20 min, 400 mPa; C: Chymotrypsin, 24 h, atmospheric pressure. Binding assays using serum from b-Lg-primed mice were performed. T cell reactivity was tested using splenocytes from b-Lg-primed mice. Mast cell degranulation in vivo was assessed by passive cutaneous anaphylaxis (PCA). Ability to trigger systemic anaphylaxis of a representative hydrolysate (A) was assessed using C3H/HeJ mice orally sensitized with b-Lg plus cholera toxin. Mice were orally challenged with 25 mg of b-Lg or hydrolysate A. Reaction severity was assessed by symptom score and body temperature after 30 min. [Results]: All hydrolysates showed a significantly reduced binding to serum from b-Lg -primed mice compared to intact b-Lg. Furthermore, hydrolysates were unable to trigger IL-13 or IFN-g release from b-Lgprimed splenocytes. The hydrolysates, unlike intact b-Lg, were unable to elicit mast cell degranulation in vivo as measured by PCA. Finally, oral challenge of b-Lg-sensitized mice with Hydrolysate A resulted in no anaphylactic symptoms compared to severe symptoms in response to b-Lg (Score 0 vs 3.4; Temperature 38.38C vs 34.78C; P<0.001). [Conclusion*: b-Lg hydrolysates produced under high hydrostatic pressure are hypoallergenic in mice and appear to be a safe and promising approach for ingredients in hypoallergenic infant formulas.
DescriptionResumen del póster presentado al Annual Meeting of American Academy of Allergy, Asthma and Immunology celebrado en San Francisco (US) del 18 al 22 de marzo de 2011.
Appears in Collections:(CIAL) Comunicaciones congresos
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