English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/114428
Título

High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta

AutorNemeckova, Ivana; Bernabéu, Carmelo ; Nachtigal, Petr
Fecha de publicación13-mar-2015
EditorPublic Library of Science
CitaciónPLoS ONE 10(3): e0119665 (2015)
ResumenIncreased levels of a soluble form of endoglin (sEng) circulating in plasma have been detected in various pathological conditions related to cardiovascular system. High concentration of sEng was also proposed to contribute to the development of endothelial dysfunction,but there is no direct evidence to support this hypothesis. Therefore, in the present work we analyzed whether high sEng levels induce endothelial dysfunction in aorta by using transgenic mice with high expression of human sEng. Transgenic mice with high expression of human sEng on CBAxC57Bl/6J background (Sol-Eng+) and age-matched transgenic littermates that do not develop high levels of human soluble endoglin (control animals in this study) on chow diet were used. As expected, male and female Sol-Eng+ transgenic mice showed higher levels of plasma concentrations of human sEng as well as increased blood arterial pressure, as compared to control animals. Functional analysis either in vivo or ex vivo in isolated aorta demonstrated that the endothelium-dependent vascular function was similar in Sol-Eng+ and control mice. In addition, Western blot analysis showed no differences between Sol-Eng+ and control mice in the protein expression levels of endoglin, endothelial NO-synthase (eNOS) and pro-inflammatory ICAM-1 and VCAM-1 from aorta. Our results demonstrate that high levels of soluble endoglin alone do not induce endothelial dysfunction in Sol-Eng+ mice. However, these data do not rule out the possibility that soluble endoglin might contribute to alteration of endothelial function in combination with other risk factors related to cardiovascular disorders.
DescripciónPetr Nachtigal et alt. 13 p.-7 fig.
Versión del editorhttp://dx.doi.org/ 10.1371/journal.pone.0119665
URIhttp://hdl.handle.net/10261/114428
DOI10.1371/journal.pone.0119665
ISSN1932-6203
E-ISSN1932-6203
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
PLOS_ONE C. Bernabeu_2015.pdf561,81 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.