English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/114403
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

L-endoglin overexpression increases renal fibrosis after unilateral ureteral obstruction

AuthorsOujo, Barbara; Langa, Carmen ; Bernabéu, Carmelo ; López-Novoa, José M.
Issue Date14-Oct-2014
PublisherPublic Library of Science
CitationPLoS ONE 9 (10): e110365 (2014)
Abstract© 2014 Oujo et al. Transforming growth factor-β (TGF-β) plays a pivotal role in renal fibrosis. Endoglin, a 180 KDa membrane glycoprotein, is a TGF-β co-receptor overexpressed in several models of chronic kidney disease, but its function in renal fibrosis remains uncertain. Two membrane isoforms generated by alternative splicing have been described, L-Endoglin (long) and S-Endoglin (short) that differ from each other in their cytoplasmic tails, being L-Endoglin the most abundant isoform. The aim of this study was to assess the effect of L-Endoglin overexpression in renal tubulo-interstitial fibrosis. For this purpose, a transgenic mouse which ubiquitously overexpresses human L-Endoglin ( L-ENG+) was generated and unilateral ureteral obstruction (UUO) was performed in L-ENG+ mice and their wild type (WT) littermates. Obstructed kidneys from L-ENG+ mice showed higher amounts of type I collagen and fibronectin but similar levels of α-smooth muscle actin (α-SMA) than obstructed kidneys from WT mice. Smad1 and Smad3 phosphorylation were significantly higher in obstructed kidneys from L-ENG+ than in WT mice. Our results suggest that the higher increase of renal fibrosis observed in L-ENG+ mice is not due to a major abundance of myofibroblasts, as similar levels of α-SMA were observed in both L-ENG+ and WT mice, but to the higher collagen and fibronectin synthesis by these fibroblasts. Furthermore, in vivo L-Endoglin overexpression potentiates Smad1 and Smad3 pathways and this effect is associated with higher renal fibrosis development.
DescriptionBarbara Oujo et alt. 12 p.-9 fig.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0110365
URIhttp://hdl.handle.net/10261/114403
DOI10.1371/journal.pone.0110365
ISSN1932-6203
E-ISSN1932-6203
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
PLOS_ONE_C. Bernabeu_2014.pdf15,2 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.