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dc.contributor.authorReina, Ramsés-
dc.contributor.authorMora, María Isabel-
dc.contributor.authorGlaria, Idoia-
dc.contributor.authorGarcía, Iker-
dc.contributor.authorSolano Goñi, Cristina-
dc.contributor.authorAmorena Zabalza, Beatriz-
dc.contributor.authorAndrés, Damián F. de-
dc.date.accessioned2015-04-27T11:35:37Z-
dc.date.available2015-04-27T11:35:37Z-
dc.date.issued2006-11-
dc.identifierdoi: 10.1016/j.virusres.2006.05.011-
dc.identifierissn: 0168-1702-
dc.identifier.citationVirus Research 121(2): 189-198 (2006)-
dc.identifier.urihttp://hdl.handle.net/10261/114280-
dc.descriptionReina, Ramsés et al.-
dc.description.abstractSmall ruminant lentiviruses (SRLV) are widely spread in many countries, including Spain. However, little is known about the genetic characteristics of Spanish goat and sheep SRLV. In this study, segments from three genomic regions (pol, gag-p25 and LTR) were amplified using DNA isolated from three Spanish autochthonous sheep (one) and goats (two). Animals (one per flock) belonged to distantly located, single-species flocks (goat or sheep). Sequence analysis showed conservation of regions that are putatively relevant to viral survival. Sequences of Spanish goat and sheep SRLV were allocated into phylogenetic trees (phylograms) with known SRLV groups. The phylograms corresponding to the pol, gag-p25 and LTR regions analyzed presented a compatible topology. This showed that Spanish caprine and ovine SRLV sequences belonged to the A or D phylogenetic groups and were closer to sheep SRLV prototypes (A1 group) than to goat SRLV prototypes (B or C groups), according to the current classification [Shah, C., Boni, J., Huder, J.B., Vogt, H.R., Muhlherr, J., Zanoni, R., Miserez, R., Lutz, H., Schupbach, J., 2004a. Phylogenetic analysis and reclassification of caprine and ovine lentiviruses based on 104 new isolates: evidence for regular sheep-to-goat transmission and worldwide propagation through livestock trade. Virology 319 (1), 12-26]. It was not possible to amplify in the three genetic regions the expected fragment in additional Spanish caprine and ovine SRLV proviral DNA sequences with the PCR primers used. This suggests that there is heterogeneity at the primer binding site among Spanish SRLV sequences. It also illustrates the need to develop diagnostic tests that are sensitive in local breeds. © 2006 Elsevier B.V. All rights reserved.-
dc.description.sponsorshipThis study was supported by grants from Spanish CICYT (AGL2000-1489-C02-01, AGL2003-08977-C03) and INIA (RTA01-107-C3-1); and the European Union (QLK2-CT-2002-00617 and EU LIFE-CRAFT No. CRAF-1999-70356). R.R. was supported by a fellowship from the Spanish CICYT. M.I.M. was supported by a grant from the Public University of Navarra.-
dc.publisherElsevier-
dc.rightsclosedAccess-
dc.subjectSpain-
dc.subjectGoats-
dc.subjectSheep-
dc.subjectCaprine Arthritis Encephalitis-
dc.subjectMaedi Visna-
dc.subjectPhylogeny-
dc.subjectpol-
dc.subjectLTR-
dc.subjectgag-
dc.subjectSmall ruminant lentiviruses-
dc.titleMolecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain-
dc.typeartículo-
dc.identifier.doi10.1016/j.virusres.2006.05.011-
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.virusres.2006.05.011-
dc.date.updated2015-04-27T11:35:38Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)-
dc.contributor.funderComisión Interministerial de Ciencia y Tecnología, CICYT (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderNafarroako Gobernua-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/100007652es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100007273es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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