English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/114280
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain

AuthorsReina, Ramsés ; Mora, María Isabel; Glaria, Idoia ; García, Iker; Solano Goñi, Cristina ; Amorena Zabalza, Beatriz ; Andrés, Damián F. de
KeywordsSpain
Goats
Sheep
Caprine Arthritis Encephalitis
Maedi Visna
Phylogeny
pol
LTR
gag
Small ruminant lentiviruses
Issue DateNov-2006
PublisherElsevier
CitationVirus Research 121(2): 189-198 (2006)
AbstractSmall ruminant lentiviruses (SRLV) are widely spread in many countries, including Spain. However, little is known about the genetic characteristics of Spanish goat and sheep SRLV. In this study, segments from three genomic regions (pol, gag-p25 and LTR) were amplified using DNA isolated from three Spanish autochthonous sheep (one) and goats (two). Animals (one per flock) belonged to distantly located, single-species flocks (goat or sheep). Sequence analysis showed conservation of regions that are putatively relevant to viral survival. Sequences of Spanish goat and sheep SRLV were allocated into phylogenetic trees (phylograms) with known SRLV groups. The phylograms corresponding to the pol, gag-p25 and LTR regions analyzed presented a compatible topology. This showed that Spanish caprine and ovine SRLV sequences belonged to the A or D phylogenetic groups and were closer to sheep SRLV prototypes (A1 group) than to goat SRLV prototypes (B or C groups), according to the current classification [Shah, C., Boni, J., Huder, J.B., Vogt, H.R., Muhlherr, J., Zanoni, R., Miserez, R., Lutz, H., Schupbach, J., 2004a. Phylogenetic analysis and reclassification of caprine and ovine lentiviruses based on 104 new isolates: evidence for regular sheep-to-goat transmission and worldwide propagation through livestock trade. Virology 319 (1), 12-26]. It was not possible to amplify in the three genetic regions the expected fragment in additional Spanish caprine and ovine SRLV proviral DNA sequences with the PCR primers used. This suggests that there is heterogeneity at the primer binding site among Spanish SRLV sequences. It also illustrates the need to develop diagnostic tests that are sensitive in local breeds. © 2006 Elsevier B.V. All rights reserved.
DescriptionReina, Ramsés et al.
Publisher version (URL)http://dx.doi.org/10.1016/j.virusres.2006.05.011
URIhttp://hdl.handle.net/10261/114280
DOI10.1016/j.virusres.2006.05.011
Identifiersdoi: 10.1016/j.virusres.2006.05.011
issn: 0168-1702
Appears in Collections:(IDAB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.