English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/114258
Título

Use of B7 costimulatory molecules as adjuvants in a prime-boost vaccination against Visna/Maedi ovine lentivirus

AutorAndrés, Ximena de ; Reina, Ramsés ; Reina, Ramsés ; Ciriza, Jesús; Crespo, Helena ; Glaria, Idoia ; Ramírez, Hugo ; Grilló, María Jesús ; Grilló, María Jesús ; Pérez, Marta M.; Andrés, Damián F. de ; Amorena Zabalza, Beatriz
Palabras claveSheep
Small ruminant lentivirus
DNA vaccination
B7 costimulatory molecules
Fecha de publicación23-jul-2009
EditorElsevier
CitaciónVaccine 27: 4591-4600 (2009)
ResumenRNA transcripts of the B7 family molecule (CD80) are diminished in blood leukocytes from animals clinically affected with Visna/Maedi virus (VMV) infection. This work investigates whether the use of B7 genes enhances immune responses and protection in immunization-challenge approaches. Sheep were primed by particle-mediated epidermal bombardment with VMV gag and env gene recombinant plasmids together with plasmids encoding both CD80 and CD86 or CD80 alone, boosted with gag and env gene recombinant modified vaccinia Ankara virus and challenged intratracheally with VMV. Immunization in the presence of one or both of the B7 genes resulted in CD4+ T cell activation and antibody production (before and after challenge, respectively), but only immunization with CD80 and CD86 genes together, and not CD80 alone, resulted in a reduced number of infected animals and increased early transient cytotoxic T lymphocytes (CTL) responses. Post-mortem analysis showed an immune activation of lymphoid tissue in challenge-target organs in those animals that had received B7 genes compared to unvaccinated animals. Thus, the inclusion of B7 genes helped to enhance early cellular responses and protection (diminished proportion of infected animals) against VMV infection. © 2009 Elsevier Ltd. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.vaccine.2009.05.080
URIhttp://hdl.handle.net/10261/114258
DOI10.1016/j.vaccine.2009.05.080
ISSN0264-410X
Aparece en las colecciones: (IDAB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Use of B7 costimulatory molecules_Andres_.pdf450,82 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.