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Development of specific diagnostic test for small ruminant lentivirus genotype E

AutorReina, Ramsés ; Grego, Elena; Profiti, Margherita; Glaria, Idoia ; Robino, Patrizia; Quasso, Antonio; Amorena Zabalza, Beatriz ; Rosati, Sergio
Palabras claveSmall ruminant lentivirus
Genotype E
Recombinant antigen
Fecha de publicación18-sep-2009
CitaciónVeterinary Microbiology 138(3-4): 251- 257 (2009)
ResumenSmall ruminant lentivirus (SRLV) belonging to the highly divergent genotype E has recently been identified in the Italian goat breed Roccaverano. In this report we have developed a specific serological test based on recombinant matrix/capsid antigen fusion protein. Performance has been evaluated and compared with a similar test based on genotype B antigen. Herds under study were selected according to the infectious status characterized by blood PCR and sequencing. Results clearly showed that B and E based recombinant ELISA only detected homologous infection and an apparent cross-reactivity was recorded in a herd in which co-infection was present. Three commercially available ELISAs showed different abilities in detecting genotype E infection, being the whole virus-based immunoassay the best choice. Genotype E-recombinant antigen was not detected in ELISA by three commercially available Mabs known to be cross-reactive among CAEV and MVV capsid antigens, further supporting the high divergence of the E genotype from others. Finally, a SRLV-free herd according to commercial ELISA testing, was analysed in the same area where genotype E was identified and few animals belonging to Roccaverano breed were found slightly reactive with the E antigens. Our results suggest that the prevalence of genotype E in other small ruminant populations may be conveniently estimated using a comparative assay based on a combination of genotype specific recombinant antigens and may highlight a wider space in which SRLVs evolve. © 2009 Elsevier B.V. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.vetmic.2009.04.005
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