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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/114176
Título

Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells

Otros títulosVinclozolin Deregulates MicroRNAs in Germ Cells
AutorBrieño-Enríquez, Miguel A.; García-López, Jesús; Cárdenas, David B.; Sylvain, Guibert; Elouan, Cleroux; Lukas, Děd; Hourcade, Juan D.; Pěknicová, Jana; Weber, Michael; Del Mazo, Jesús
Palabras claveVenclozolin
MicroRNAs
Germ Cells
Transgenerational effects
Epigenetics
Fecha de publicación21-abr-2015
EditorPublic Library of Science
CitaciónPLoS ONE 10(4): e0124296
ResumenIn mammals, germ cell differentiation is initiated in the Primordial Germ Cells (PGCs) during fetal development. Prenatal exposure to environmental toxicants such as endocrine disruptors may alter PGC differentiation, development of the male germline and induce transgenerational epigenetic disorders. The anti-androgenic compound vinclozolin represents a paradigmatic example of molecule causing transgenerational effects on germ cells. We performed prenatal exposure to vinclozolin in mice and analyzed the phenotypic and molecular changes in three successive generations. A reduction in the number of embryonic PGCs and increased rate of apoptotic cells along with decrease of fertility rate in adult males were observed in F1 to F3 generations. Blimp1 is a crucial regulator of PGC differentiation. We show that prenatal exposure to vinclozolin deregulates specific microRNAs in PGCs, such as miR-23b and miR-21, inducing disequilibrium in the Lin28/let-7/Blimp1 pathway in three successive generations of males. As determined by global maps of cytosine methylation, we found no evidence for prominent changes in DNA methylation in PGCs or mature sperm. Our data suggest that embryonic exposure to environmental endocrine disruptors induces transgenerational epigenetic deregulation of expression of microRNAs affecting key regulatory pathways of germ cells differentiation.
Descripción19 p.-6 fig.
Versión del editorhttp://dx.doi.org/10.1371/journal.pone.0124296
URIhttp://hdl.handle.net/10261/114176
DOI10.1371/journal.pone.0124296
ISSN1932-6203
E-ISSN1932-6203
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