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dc.contributor.authorVolkmer, Tiago-
dc.contributor.authorMagalhães, Joana-
dc.contributor.authorSousa, Vania-
dc.contributor.authorSantos, Luis A.-
dc.contributor.authorBurguera, Elena F.-
dc.contributor.authorBlanco, Francisco J.-
dc.contributor.authorSan Román, Julio-
dc.contributor.authorRodríguez-Lorenzo, Luis M.-
dc.identifierdoi: 10.3390/polym6102510-
dc.identifierissn: 2073-4360-
dc.identifier.citationPolymers 6: 2510-2525 (2014)-
dc.description.abstractThe aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.-
dc.description.sponsorshipWe acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI)-
dc.publisherMultidisciplinary Digital Publishing Institute-
dc.subjectPDMAEMA (Poly(dimethyl aminoethyl methacrylate))-
dc.subjectPHEMA (poly(hydroxyethyl methacrylate)-
dc.subjectMesenchymal stem cells-
dc.subjectα-tricalcium phosphate-
dc.title2-(dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells-
dc.description.versionPeer Reviewed-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
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