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2-(dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells

AuthorsVolkmer, Tiago; Magalhães, Joana; Sousa, Vania; Santos, Luis A.; Burguera, Elena F.; Blanco, Francisco J. ; San Román, Julio ; Rodríguez-Lorenzo, Luis M.
KeywordsPDMAEMA (Poly(dimethyl aminoethyl methacrylate))
PHEMA (poly(hydroxyethyl methacrylate)
Mesenchymal stem cells
α-tricalcium phosphate
Issue Date2014
PublisherMultidisciplinary Digital Publishing Institute
CitationPolymers 6: 2510-2525 (2014)
AbstractThe aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.
Identifiersdoi: 10.3390/polym6102510
issn: 2073-4360
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