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Smt3 is required for ecdysone synthesis in Drosophila melanogaster

AuthorsTalamillo, Ana; Sánchez, Jonatan; Cantera, R.; Pérez, Coralia; Martín Casacuberta, David A. ; Barrio, Rosa
Issue DateJul-2008
Citation17th International Ecdysone Workshop (2008)
AbstractTo study in vivo the role of the ubiquitin-like protein Smt3 (Sumo) during Drosophila development, we generated transgenic flies carrying the transgene UAS-smt3i to reduce smt3 mRNA levels in specific groups of cells. Low smt3 in the prothoracic gland prevents metamorphosis. RNAi knockdown larvae stop their development in their last larval stage and remain alive for up to a month, during which they continue to eat and gain weight. Their prothoracic glands have fewer, but larger cells than normal. They also have lower ecdysteroid titre than WT. After dietary administration of exogenous ecdysone these larvae form pupal cases, but do not proceed further in development and die. We observed that, in larvae with lower levels of smt3, the subcellular localization and expression levels of factors involved in the regulation of ecdysteroids synthesis, are altered. These results are in concordance to larvae mutant in lesswright, the homologue of the Sumo conjugating enzyme gene Ubc9. Interestingly, prothoracic gland cells present reduced intracellular channels and a reduced content in lipid droplets and cholesterol, which could contribute to the deficit in steroidogenesis.
DescriptionTrabajo presentado en el 17th International Ecdysone Workshop, celebrado en Ulm (Alemania) del 20 al 24 de junio de 2008.
Appears in Collections:(IBE) Comunicaciones congresos
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