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Title

Comparison of the activity of ecdysone agonists in Bombyx mori and Spodoptera littoralis by in vitro reporter assays and in vivo toxicity assays

AuthorsSoin, Thomas; Mosallanejad, H.; Martín Casacuberta, David A. ; Latrou, K.; Nakagawa, Y.; Smagghe, G.; Swevers, Luc
Issue DateJul-2008
Citation17th International Ecdysone Workshop (2008)
AbstractEcdysone agonists such as dibenzoylhydrazines have been successfully used in biological control programs of lepidopteran and coleopteran pests. However, little information exists with respect of the specificity of the action of such compounds towards different species of Lepidoptera or Coleoptera. In this study, we compare the activity of ecdysone agonists belonging to three different chemical classes (dibenzoylhydrazines, acylaminoketones and tetrahydroquinolines) between the lepidopteran pest Spodoptera littoralis and a beneficial lepidopteran species, the silkworm Bombyx mori. The activity of ecdysteroid agonists is measured both in vitro, using an ecdysone-responsive reporter assay based on transfected or transformed Spodoptera- or Bombyx-derived cell lines (Sl2 and Bm5 cells, respectively), and in vivo, by toxicity tests on Spodoptera and Bombyx larvae. The study aims at the elucidation of the species-specific factors that determine the activity of ecdysone agonists against Lepidoptera.
DescriptionTrabajo presentado en el 17th International Ecdysone Workshop, celebrado en Ulm (Alemania) del 20 al 24 de junio de 2008.
URIhttp://hdl.handle.net/10261/114048
Appears in Collections:(IBMB) Comunicaciones congresos
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