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Functional analysis of the nuclear receptor seven-up during nymphal and adult development in the hemimetabolan insect Blattella germanica

AuthorsBorràs Castells, Ferran ; Bellés, Xavier ; Martín Casacuberta, David A.
Issue DateJul-2010
Citation18th International Ecdysone Workshop (2010)
AbstractIn insects, major developmental transitions occurring throughout their life cycles are tightly controlled by changes in the titer of the ecdysteroidal hormone, 20-hydroxyecdysone (20E). 20E regulates these transitions upon binding to its heterodimeric receptor formed by two nuclear hormone receptors, EcR and the RXR homolog USP. Moreover, the activated receptor elicits cascades of gene expression that mediate and amplify the hormonal signal. Molecular characterization of most of these genes revealed that they encode transcription factors, such as E75, E74 and Broad-Complex. However, throughout development the precise timing of the 20E responses requires not only the positive actitivity of the 20E-dependent factors but also the participation of negative transcriptional regulators. In Drosophila melanogaster and Aedes aegypti, two holometabolan insects, the nuclear receptor seven-up attenuates the activity of the 20E-signalling pathway by inhibiting the normal functioning of the EcR-USP/RXR heterodimer. In order to analyze whether this repressive effect is conserved in less modified hemimetabolan insects, we have cloned two isoforms of seven-up (BgSvp-A and BgSvp-B) of the German cockroach Blattella germanica. We are currently examining the role of both isoforms employing a RNAi in vivo approach to determine which processes are regulated by this nuclear receptor during nymphal and adult development.
DescriptionPóster presentado en el 18th International Ecdysone Workshop, celebrado del 19 al 23 de julio en Ceske Budejovice (República Checa).
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