English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/113959
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Yeast genetic analysis reveals the involvement of chromatin reassembly factors in repressing HIV-1 basal transcription

AuthorsVanti, Manuela; Gallastegui, Edurne; Respaldiza, Iñaki; Rodríguez-Gil, Alfonso; Gómez-Herreros, Fernando ; Jimeno-González, Silvia ; Jordan, Albert ; Chávez, Sebastián
Issue Date16-Jan-2009
PublisherPublic Library of Science
CitationPLoS Genetics 5(1):e1000339 (2009)
AbstractRebound of HIV viremia after interruption of anti-retroviral therapy is due to the small population of CD4+ T cells that remain latently infected. HIV-1 transcription is the main process controlling post-integration latency. Regulation of HIV-1 transcription takes place at both initiation and elongation levels. Pausing of RNA polymerase II at the 5′ end of HIV-1 transcribed region (5′HIV-TR), which is immediately downstream of the transcription start site, plays an important role in the regulation of viral expression. The activation of HIV-1 transcription correlates with the rearrangement of a positioned nucleosome located at this region. These two facts suggest that the 5′HIV-TR contributes to inhibit basal transcription of those HIV-1 proviruses that remain latently inactive. However, little is known about the cell elements mediating the repressive role of the 59HIV-TR. We performed a genetic analysis of this phenomenon in Saccharomyces cerevisiae after reconstructing a minimal HIV-1 transcriptional system in this yeast. Unexpectedly, we found that the critical role played by the 5′HIV-TR in maintaining low levels of basal transcription in yeast is mediated by FACT, Spt6, and Chd1, proteins so far associated with chromatin assembly and disassembly during ongoing transcription. We confirmed that this group of factors plays a role in HIV-1 postintegration latency in human cells by depleting the corresponding human orthologs with shRNAs, both in HIV latently infected cell populations and in particular single-integration clones, including a latent clone with a provirus integrated in a highly transcribed gene. Our results indicate that chromatin reassembly factors participate in the establishment of the equilibrium between activation and repression of HIV-1 when it integrates into the human genome, and they open the possibility of considering these factors as therapeutic targets of HIV-1 latency. © 2009 Vanti et al.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pgen.1000339
http://dx.doi.org/10.1371/annotation/45587774-5d7c-4023-97b0-96833be7ad9a
URIhttp://hdl.handle.net/10261/113959
DOI10.1371/annotation/45587774-5d7c-4023-97b0-96833be7ad9a
Identifiersdoi: 10.1371/journal.pgen.1000339
issn: 1553-7404
Appears in Collections:(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
Vanti-PLoS-Genetics-2009-v5-n1-e1000339.pdf941 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.