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Determinants of the specificity of human histone H1 subtypes

Autor Jordan, Albert
Fecha de publicación 4-mar-2013
Citación III Annual Chromatin and Epigenetics Symposium (2013)
ResumenSeven linker histone H1 variants exist in human somatic cells (H1.0, H1.1 to H1.5, and H1X), with distinct prevalence depending on the cell type analyzed and along differentiation, that bind to linker DNA contributing to higher order chromatin compaction. In addition, H1 seems to be actively involved in the regulation of gene expression. It is not well known whether the different variants have specific roles or regulate specific promoters. We have explored this by inducible shRNA-mediated knock-down of each of the H1 variants in a human breast cancer cell line. Rapid inhibition of each H1 variant was not compensated by changes of expression of other variants. Thus, specific phenotypes are observed in breast cancer cells depleted of individual histone H1 variants. Moreover, knock-down of each H1 variant alters expression of a different, reduced subset of genes, with more genes being repressed than activated suggesting a local positive role of H1 on gene expression control. By taking advantage of specific antibodies for H1 variants and HA-tagged recombinant H1 variants-expressing cell lines, we have investigated the distribution in particular promoters and genome-wide of the different H1 variants, as well as the role of some H1 post-translational modifications. Finally, we have explored changes on the histone H1 variants content during differentiation of human embryonic stem cells and reprogramming of keratinocytes
Descripción Comunicación presentada en el III Annual Chromatin and Epigenetics Symposium, celebrado el 4 de marzo de 2014 en Barcelona (España)
URI http://hdl.handle.net/10261/113928
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