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Evolution of SUMO protein functions in insects

AuthorsUreña, Enric ; Pérez, Coralia; Rodríguez, Manuel S.; Lang, Valérie; Pirone, Lucia; Sutherland, James D.; Barrio, Rosa; Martín Casacuberta, David A.
Issue DateJul-2013
CitationInsect Hormones (19th Ecdysone) International Workshop (2013)
AbstractSUMOylation is a highly conserved post-translational modification that modulates target protein activity in a great number of cellular processes during development. In the holometabolous insect Drosophila melanogaster, SUMOylation has been shown to be necessary during the metamorphic transition. Reduction of the single D. melanogaster SUMO homologue (smt3) expression in the prothoracic gland prevents the larval to pupal transition due to reduced lipid content in the gland and low ecdysone titer1. Unfortunately, our knowledge on the role of SUMOylation in insect development exclusively derives from studies in D. melanogaster, which shows a highly derived type of insect development that may not be representative in the insect class context. Our research, therefore, is devoted to establish the role of SUMOylation in the hemimetabolous insect Blattella germanica. In contrast to D. melanogaster, we have identified two B. germanica SUMO homologs, BgSumo1 and BgSumo3 that are ubiquitously expressed throughout development. By using RNAi in vivo experiments we have shown that, whereas BgSumo3 is dispensable for the correct development of B. germanica, reduction of BgSumo1 levels resulted in severe defects during the metamorphic transition, including a marked developmental delay due to impaired activation of the ecdysone-triggered signaling cascade. Furthermore, we have shown that all the proteins belonging to the ecdysone-dependent transcriptional cascade of nuclear hormone receptors (BgEcR, BgRXR, BgE75, BgHR3 and BgFTZ-F1) are SUMOylated in vitro. Finally, to test whether the functions of SUMO proteins are evolutionary conserved between hemimetabolous and holometabolous insects, we performed functional analysis in vivo of the two B. germanica Sumo homologues using D. melanogaster as model system. These experiments showed that BgSumo3 contains all the elements necessary to substitute functionally for D. melanogaster smt3, while BgSumo1 does not.
DescriptionPóster presentado en el Insect Hormones (19th Ecdysone) International Workshop, celebrado en Minneapolis del 21 al 26 de julio de 2013.
Appears in Collections:(IBE) Comunicaciones congresos
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