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Title

Unique crystal structure of a novel surfactant protein from the foam nest of the frog leptodactylus vastus

AuthorsCavalcante Hissa, Denise; Arrusa Bezerra, Gustavo; Birner-Gruenberger, Ruth; Paulino Silva, Luciano; Usón, Isabel ; Gruber, Karl; Maciel Melo, Vania Maria
Keywordsamphibians
Lv-ranaspumin
mass spectrometry
structural biology
surfactants
Issue Date10-Feb-2014
PublisherJohn Wiley & Sons
CitationChemBioChem 15(3): 393- 398 (2014)
AbstractBreeding by releasing eggs into stable biofoams (>foam nests>) is a peculiar reproduction mode within anurans, fish, and tunicates; not much is known regarding the biochemistry or molecular mechanisms involved. Lv-ranaspumin (Lv-RSN-1) is the predominant protein from the foam nest of the frog Leptodactylus vastus. This protein shows natural surfactant activity, which is assumed to be crucial for stabilizing foam nests. We elucidated the amino acid sequence of Lv-RSN-1 by de novo sequencing with mass-spectrometry and determined the high-resolution X-ray structure of the protein. It has a unique fold mainly composed of a bundle of 11 α-helices and two small antiparallel β-strands. Lv-RSN-1 has a surface rich in hydrophilic residues and a lipophilic cavity in the region of the antiparallel β-sheet. It possesses intrinsic surface-active properties, reducing the surface tension of water from 73 to 61 mN m-1 (15 μg mL-1). Lv-RSN-1 belongs to a new class of surfactants proteins for which little has been reported regarding structure or function. Making the perfect egg: Frog eggs and sperm are matured in >foam nests> that are stabilized by the naturally occurring surfactant protein ranaspumin. We report the amino acid sequence, crystal structure (which corresponds to a novel fold), and biochemical properties of Lv-RSN-1, the uncommon ranaspumin from the frog Leptodactylus vastus. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publisher version (URL)http://dx.doi.org/10.1002/cbic.201300726
URIhttp://hdl.handle.net/10261/113924
DOI10.1002/cbic.201300726
Identifiersdoi: 10.1002/cbic.201300726
issn: 1439-4227
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