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Title

miR-127-5p targets the 3'UTR of human â-F1-ATPase mRNA and inhibits its translation

AuthorsWillers, Imke M. ; Martínez-Reyes, Inmaculada ; Martínez-Díez, Marta; Cuezva, José M.
KeywordsLiver
H+ATP synthase
Translational control
Human development
Pathology
Mitochondria
Issue Date2012
PublisherElsevier
CitationBiochimica et Biophysica Acta - Bioenergetics 1817: 838- 848 (2012)
AbstractThe mitochondrial H+ATP synthase is a bottleneck component in the provision of metabolic energy by oxidative phosphorylation. The expression of its catalytic subunit (β-F1-ATPase) is stringently controlled at post-transcriptional levels during oncogenesis, the cell cycle and in development. Here we show that miR-127-5p targets the 3 UTR of β F1-ATPase mRNA (β-mRNA) significantly reducing its translational efficiency without affecting β mRNA abundance. Despite the reduced expression of βF1-ATPase in most human carcinomas, we observed no expression of miR-127-5p in different human cancer cell lines, minimizing the potential role of miR-127-5p as a regulator of the bioenergetic activity of mitochondria in cancer. In contrast, miR 127-5p is highly over-expressed in the human fetal liver. Consistent with previous findings in the rat, the expression of β F1-ATPase in the human liver also seems to be controlled at post-transcriptional levels during development, what might suggest a role for miR-127-5p in controlling β mRNA translation and thus in de fining the bioenergetic activity of human liver mitochondria. Moreover, immunolocalization techniques and subcellular fractionation experiments using different antibodies against β F1-ATPase reveal that the ectopic expression of β F1-ATPase at the cell surface of the hepatocytes and HepG2 cells is negligible or stands for scrutiny
URIhttp://hdl.handle.net/10261/113921
DOIhttp://dx.doi.org/10.1016/j.bbabio.2012.03.005
Identifiersdoi: doi: 10.1016/j.bbabio.2012.03.005
issn: 0005-2728
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