English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/113921
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 17 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)

miR-127-5p targets the 3'UTR of human â-F1-ATPase mRNA and inhibits its translation

Autor Willers, Imke M.; Martínez-Reyes, Inmaculada; Martínez-Díez, Marta; Cuezva, José M.
Palabras clave Liver
H+ATP synthase
Translational control
Human development
Fecha de publicación 2012
Citación Biochimica et Biophysica Acta - Bioenergetics 1817: 838- 848 (2012)
ResumenThe mitochondrial H+ATP synthase is a bottleneck component in the provision of metabolic energy by oxidative phosphorylation. The expression of its catalytic subunit (β-F1-ATPase) is stringently controlled at post-transcriptional levels during oncogenesis, the cell cycle and in development. Here we show that miR-127-5p targets the 3 UTR of β F1-ATPase mRNA (β-mRNA) significantly reducing its translational efficiency without affecting β mRNA abundance. Despite the reduced expression of βF1-ATPase in most human carcinomas, we observed no expression of miR-127-5p in different human cancer cell lines, minimizing the potential role of miR-127-5p as a regulator of the bioenergetic activity of mitochondria in cancer. In contrast, miR 127-5p is highly over-expressed in the human fetal liver. Consistent with previous findings in the rat, the expression of β F1-ATPase in the human liver also seems to be controlled at post-transcriptional levels during development, what might suggest a role for miR-127-5p in controlling β mRNA translation and thus in de fining the bioenergetic activity of human liver mitochondria. Moreover, immunolocalization techniques and subcellular fractionation experiments using different antibodies against β F1-ATPase reveal that the ectopic expression of β F1-ATPase at the cell surface of the hepatocytes and HepG2 cells is negligible or stands for scrutiny
URI http://hdl.handle.net/10261/113921
Identificadoresdoi: doi: 10.1016/j.bbabio.2012.03.005
issn: 0005-2728
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.