English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/113894
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 1 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

High crystallizability under air-exclusion conditions of the full-length LysR-type transcriptional regulator TsaR from Comamonas testosteroni T-2 and data-set analysis for a MIRAS structure-solution approach

Autor Monferrer, Dominique; Tralau, Tewes; Kertesz, Michael; Panjikar, Santosh; Usón, Isabel
Palabras clave Microbatch crystallization
Full-length LysR-type regulator
MIRAS phasing
Fecha de publicación 1-sep-2008
EditorInternational Union of Crystallography
Citación Acta Crystallographica Section F: Structural Biology and Crystallization Communications 64(8): 764-769 (2008)
ResumenThe full-length LysR-type transcriptional regulator TsaR from Comamonas testosteroni T-2 was heterologously overexpressed in Escherichia coli, purified and stabilized under conditions that favoured its rapid crystallization using the microbatch-under-oil technique. The purified protein was highly crystallizable and two different crystal forms were readily obtained. However, only monoclinic crystals gave diffraction beyond 2 Å and there was a slight variation in unit-cell parameters between crystals. The only other LysR-type regulator for which a full-length crystal form is available is CbnR, but no solution could be obtained when this was used as a model in molecular replacement. Mercury and xenon derivatives were therefore produced in order to phase the structure using a MIRAS approach. © International Union of Crystallography 2008.
Versión del editorhttp://dx.doi.org/10.1107/S1744309108019738
URI http://hdl.handle.net/10261/113894
DOI10.1107/S1744309108019738
Identificadoresdoi: 10.1107/S1744309108019738
issn: 1744-3091
Aparece en las colecciones: (IBMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Monferrer-Acta-Crystallographica-F-2008-v64-n8-p764.pdf683,56 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.