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Título

Metabolomic study of human cerebrospinal fluid using CE-MS

Autor Ibáñez, Clara ; Simó, Carolina ; Cedazo-Minguez, Angel; Cifuentes, Alejandro
Fecha de publicación 2010
Citación MSB 2010
ResumenIn this work, capillary electrophoresis-mass spectrometry (CE-MS) is used to carry out a metabolomic study of human cerebrospinal fluid (CSF). A CE-MS coupling with an electrospray sheath-liquid interface (ESI) and a time of flight (TOF) MS analyzer were used in this work. Due to the high scan speed and high mass resolution of TOF MS, this analyzer is well-suited for on-line coupling with fast separation techniques as CE to analyze complex samples. TOF MS also provides high mass accuracy determination, essential to obtain as much information as possible from complex biological fluids as CSF. The CSF was analyzed with minimal sample pre-treatment: deproteinization was carried out by using ultracentrifugation and a membrane with 3000 Da cutt-off. The ultrafiltrate was directly injected to the CE-MS. For the metabolic profiling of human CSF by CE-ESI-TOF MS an acidic volatile buffer was used together with a bare fused-silica capillary. Reproducible analyses were obtained with %RSD values within the same day (five consecutive injections) lower than 0.5 % and 4 % for analysis time and peak area, respectively. A metabolic profile in the positive ionization mode was obtained in less than 30 min. After discarding the potential interferences from solvent ions, adducts and other contaminants, a total number of 120 peaks were subjected to peak identification. The high mass accuracy provided by TOF MS allowed generating a molecular formula for each metabolite that was then used for metabolite identification using HDBM, Metlin, Kegg, Chemspider, PubChem, etc. databases. Tentative identification of more than 80 compounds is achieved in this work, to our knowledge, the highest number of compounds identified in human CSF by CE-MS so far. The potential of this CE-MS metabolomic approach will be used to detect biomarkers of a variety of neurological diseases.
Descripción Resumen del póster presentado al 25th International Symposium on Microscale BioSeparations celebrado en Praga (Republica Checa) del 21 al 25 de marzo de 2010.
URI http://hdl.handle.net/10261/113802
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