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Título: | Synthesis and characterization of isomaltulose-derived oligosaccharides produced by transglucosylation reaction of Leuconostoc mesenteroides dextransucrase |
Autor: | Barea-Alvarez, Montserrat CSIC; Benito, Maria Teresa CSIC ORCID; Olano, Agustín CSIC ; Jimeno, M. Luisa CSIC ORCID; Moreno, F. Javier CSIC ORCID | Palabras clave: | Bioactive oligosaccharides Transglycosylation Dextransucrase Leuconostoc mesenteroides Isomaltulose |
Fecha de publicación: | 2014 | Editor: | American Chemical Society | Citación: | Journal of Agricultural and Food Chemistry 62(37): 9137-9144 (2014) | Resumen: | This paper reports the efficient enzymatic synthesis of a homologous series of isomaltulose-derived oligosaccharides with degrees of polymerization ranging from 3 to 9 through the transglucosylation reaction using a dextransucrase from Leuconostoc mesenteroides B-512F. The total oligosaccharide yield obtained under optimal conditions was 41–42% (in weight with respect to the initial amount of isomaltulose) after 24–48 h of reaction. Nuclear magnetic resonance (NMR) structural characterization indicated that dextransucrase specifically transferred glucose moieties of sucrose to the C-6 of the nonreducing glucose residue of isomaltulose. Likewise, monitoring the progression of the content of each individual oligosaccharide indicated that oligosaccharide acceptor products of low molecular weight acted in turn as acceptors for further transglucosylation to yield oligosaccharides of a higher degree of polymerization. The produced isomaltulose-derived oligosaccharides can be considered as isomalto-oligosaccharides (IMOs) because they are linked by only α-(1→6) bonds. In addition, having isomaltulose as the core structure, these IMO-like structures could possess appealing bioactive properties that could find potential applications as functional food ingredients. | Versión del editor: | http://dx.doi.org/10.1021/jf5033735 | URI: | http://hdl.handle.net/10261/113652 | DOI: | 10.1021/jf5033735 | Identificadores: | doi: 10.1021/jf5033735 issn: 0021-8561 e-issn: 1520-5118 |
Aparece en las colecciones: | (CIAL) Artículos (CENQUIOR) Artículos |
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