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Título

Isolation and characterization of extrachromosomal double-stranded RNA elements in xanthophyllomyces dendrorhous

Autor Baeza, Marcelo; Fernández Lobato, María; Cifuentes, Víctor
Palabras clave Xanthophyllomyces dendrorhous
Mycovirus
Astaxanthin
DsRNA
Fecha de publicación 2012
EditorHumana Press
Citación Methods in molecular biology (Clifton, N,J,) 898: 195- 205 (2012)
ResumenDouble-stranded RNA (dsRNA) molecules are widely found in yeasts and filamentous fungi. It has been suggested that may play important roles in the evolution of eukaryote genomes and may be a valuable tool in yeast typing. The characterization of these extrachromosomal genetic elements is usually a laborious process, especially when trying to analyze a large number of samples. In this chapter, we describe a simple method to isolate dsRNA elements from yeasts using low amounts of starting material, and their application to different Xanthophyllomyces dendrorhous strains. Furthermore, the methodologies for enzymatic and hybridization characterizations, and quantification of relative dsRNA abundance are detailed. © 2012 Springer Science+Business Media New York.
URI http://hdl.handle.net/10261/113648
DOI10.1007/978-1-61779-918-1_13
Identificadoresdoi: 10.1007/978-1-61779-918-1_13
issn: 1064-3745
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