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Título: | Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast |
Autor: | López Malo, María; García Ríos, Estéfani CSIC ORCID; Martí Raga, María; Chiva, Rosa Ana CSIC ORCID CVN; Guillamón, José Manuel CSIC ORCID | Palabras clave: | Cold Wine Yeast Nitrogen |
Fecha de publicación: | jul-2014 | Editor: | American Institute of Chemical Engineers | Citación: | Biotechnology Progress 30 (4): 776-783 (2014) | Resumen: | Low-temperature fermentations produce wines with greater aromatic complexity, but the success of these fermentations greatly depends on the adaptation of yeast cells to cold. Tryptophan has been previously reported to be a limiting amino acid during Saccharomyces cerevisiae growth at low temperature. The objective of this study was to determine the influence of the tryptophan metabolism on growth and fermentation performance during low-temperature wine fermentation. To this end, we constructed the deletion mutants of the TRP1 and TAT2 genes in a derivative haploid of a commercial wine strain, and the TAT2 gene was overexpressed in the prototroph and auxotroph (Δtrp1) backgrounds. Then we characterized growth and fermentation activity during wine fermentation at low and optimum temperatures. Our results partially support the role of this amino acid in cold yeast growth. Although deletion of TRP1 impaired amino acid uptake and the growth rate at low temperature in synthetic must, this growth impairment did not affect the fermentation rate. Deletion of TAT2 endorsed this strain with the highest nitrogen consumption capacity and the greatest fermentation activity at low temperature. | Descripción: | 8 pages, 3 figures, 4 tables.-- First published online: 30 APR 2014 | Versión del editor: | http://dx.doi.org/10.1002/btpr.1915 | URI: | http://hdl.handle.net/10261/113486 | DOI: | 10.1002/btpr.1915 | ISSN: | 8756-7938 |
Aparece en las colecciones: | (IATA) Artículos |
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