Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/113450
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Schultheiss, Kira P. | - |
dc.contributor.author | Suga, Hiroshi | - |
dc.contributor.author | Ruiz-Trillo, Iñaki | - |
dc.contributor.author | Miller, W. Todd | - |
dc.date.accessioned | 2015-04-09T12:30:47Z | - |
dc.date.available | 2015-04-09T12:30:47Z | - |
dc.date.issued | 2012-09-21 | - |
dc.identifier | doi: 10.1021/bi300965h | - |
dc.identifier | issn: 0006-2960 | - |
dc.identifier | e-issn: 1520-4995 | - |
dc.identifier.citation | Biochemistry 51(41): 8267-8277 (2012) | - |
dc.identifier.uri | http://hdl.handle.net/10261/113450 | - |
dc.description | PMCID: PMC3567254 | - |
dc.description.abstract | Phosphotyrosine-based signaling plays a vital role in cellular communication in multicellular organisms. Unexpectedly, unicellular choanoflagellates (the closest phylogenetic group to metazoans) possess numbers of tyrosine kinases that are comparable to those in complex metazoans. Here, we have characterized tyrosine kinases from the filasterean Capsaspora owczarzaki, a unicellular protist representing the sister group to choanoflagellates and metazoans. Two Src-like tyrosine kinases have been identified in C. owczarzaki (CoSrc1 and CoSrc2), both of which have the arrangement of SH3, SH2, and catalytic domains seen in mammalian Src kinases. In Capsaspora cells, CoSrc1 and CoSrc2 localize to punctate structures in filopodia that may represent primordial focal adhesions. We have cloned, expressed, and purified both enzymes. CoSrc1 and CoSrc2 are active tyrosine kinases. Mammalian Src kinases are normally regulated in a reciprocal fashion by autophosphorylation in the activation loop (which increases activity) and by Csk-mediated phosphorylation of the C-terminal tail (which inhibits activity). Similar to mammalian Src kinases, the enzymatic activities of CoSrc1 and CoSrc2 are increased by autophosphorylation in the activation loop. We have identified a Csk-like kinase (CoCsk) in the genome of C. owczarzaki. We cloned, expressed, and purified CoCsk and found that it has no measurable tyrosine kinase activity. Furthermore, CoCsk does not phosphorylate or regulate CoSrc1 or CoSrc2 in cells or in vitro, and CoSrc1 and CoSrc2 are active in Capsaspora cell lysates. Thus, the function of Csk as a negative regulator of Src family kinases appears to have arisen with the emergence of metazoans. © 2012 American Chemical Society. | - |
dc.description.sponsorship | Work in W.T.M.’s laboratory was supported by Grant CA58530 from the National Institutes of Health. Work in I.R.-T.’s lab was supported by a European Research Council Starting Grant (ERC-2007-StG-206883) and a grant (BFU2008-02839/BMC) from Ministerio de Ciencia e Innovación (MICINN). | - |
dc.publisher | American Chemical Society | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/206883 | - |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | - |
dc.title | Lack of Csk-mediated negative regulation in a unicellular Src kinase | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1021/bi300965h | - |
dc.relation.publisherversion | http://dx.doi.org/10.1021/bi300965h | - |
dc.date.updated | 2015-04-09T12:30:49Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | National Institutes of Health (US) | - |
dc.contributor.funder | European Research Council | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100000002 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000781 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.pmid | 22998693 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
Aparece en las colecciones: | (IBE) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
lack_Csk-mediated_Schultheiss.pdf | 4,31 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
15
checked on 31-mar-2024
SCOPUSTM
Citations
19
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
18
checked on 25-feb-2024
Page view(s)
301
checked on 17-abr-2024
Download(s)
319
checked on 17-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.